Gingko diterpene lactone composition
A technology of ginkgo diterpene lactones and ginkgolides, which is applied in the field of ginkgo diterpene lactone compositions, and can solve problems such as research on the ratio relationship of diterpene lactones
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Embodiment 1
[0024] Example 1 Protective effect of ginkgo diterpene lactone composition on ischemic brain disease model
[0025] 1. Materials
[0026] 1.1 The ginkgo diterpene lactone composition can be prepared by the following methods: get ginkgo biloba through ethanol extraction, concentration, adsorbent impurity removal, organic solvent elution, washing and purification, steps such as recrystallization, and extract parameters, adsorbent wherein Selection, eluent selection, washing solution selection and recrystallization solvent selection are adjusted to obtain; or prepared according to the method disclosed in CN1424031A, and prepared by adjusting the preparation parameters; the composition can also be prepared by compounding the compound And get. Obtain the ginkgo diterpene lactone composition of Table 1 proportioning by above-mentioned method.
[0027] Obtain different compositions or extracts by weight percentage by each method above, and measure the percentage content of its gink...
Embodiment 2
[0062] Example 2 Effect of Ginkgo Diterpene Lactone Composition on Platelet Aggregation in Rabbits
[0063] 1. Experimental materials
[0064] 1.1 Instrument
[0065] STEELIEX platelet aggregation coagulation factor analyzer, Beijing Shidi Scientific Instrument Company; LDZ5-2 centrifuge, Beijing Medical Centrifuge Factory.
[0066] 1.2 Reagents and Drugs
[0067] The specific ratio of the drug group is the same as in Table 1.
[0068] Nimodipine, produced by Shandong Xinhua Pharmaceutical Co., Ltd., batch number 1609215; adenosine diphosphate (adenosindiphosphate, ADP), Shanghai Boao Biotechnology Co., Ltd. (imported packaging); platelet activating factor (platelet activating factor, PAF), by sigma Provided by the company; trisodium citrate, Shanghai Lingfeng Chemical Reagent Co., Ltd.
[0069] 1.3 Experimental animals
[0070] New Zealand white rabbits were provided by Laifu Farm, Pukou District, Nanjing, with the experimental animal production license SCXK (Su) 2014-00...
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