A kind of isoflavones extracted from papaya and its preparation method and application

A technology of isoflavones and compounds, applied in the field of phytochemistry, can solve problems that have not been seen in the application, and achieve the effects of easy popularization and application, good antioxidant activity, and good free radical scavenging activity

Active Publication Date: 2020-08-25
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The present invention isolates a kind of isoflavone compound with good antioxidant activity from papaya, a traditional medicine and food homologous plant, and no related reports have been seen on the compound and its application in inhibiting the oxidative deterioration of spices

Method used

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  • A kind of isoflavones extracted from papaya and its preparation method and application
  • A kind of isoflavones extracted from papaya and its preparation method and application
  • A kind of isoflavones extracted from papaya and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] The papaya sample was taken from Linyi, Shandong Province, and the variety was Yizhou papaya. Take 5.2kg of dried papaya fruit, coarsely crush it to 30 mesh, extract it four times with 70% acetone ultrasonically, each time for 40 minutes, and combine the extracts; filter the extracts , concentrated under reduced pressure to 1 / 4 of the volume; let stand, filter out the precipitate, and concentrate into 234g of extract; add 500g of acetone to the extract to dissolve, then add 300g of 100 mesh silica gel to mix the sample, after mixing the sample, use 1.2 kg of 200 mesh silica gel was packed into the column; the gradient elution was carried out with chloroform-acetone mixed organic solvents with volume ratios of 9:1, 8:2, 7:3, 6:4, 1:1, and 0:1, and the gradient elution was collected. Deliquification, concentration, monitored by TLC, and the same parts were combined to obtain 6 parts A-F, wherein, for the collected sample D (6:4) part 31.4g, 50% methanol was used as the mob...

Embodiment 2

[0040] The papaya sample was taken from Nanyang, Henan Province, and the variety Tongbai papaya was 6.5kg of dried papaya, coarsely crushed to 35 mesh, ultrasonically extracted 4 times with 70% acetone, 50 minutes each time, and the extracts were combined; the extracts were filtered , concentrated under reduced pressure to 1 / 4 of the volume; let stand, filter out the precipitate, and concentrate into 220g of extract; add 405g of acetone to the extract to dissolve, then add 440g of 100 mesh silica gel to mix the sample, after mixing the sample, use 200-mesh silica gel 1.8kg column; gradient elution with chloroform-acetone mixed organic solvents with volume ratios of 9:1, 8:2, 7:3, 6:4, 1:1 and 0:1 respectively, and the gradient elution Deliquification, concentration, monitored by TLC, combined the same parts to obtain 6 parts A-F, wherein, for the collected sample D (6:4) part 65.8g, then use 50% methanol as mobile phase, flow rate 20ml / min, 21.2×250mm, 5μm ZorbaxPrepHT GF rev...

Embodiment 3

[0042] The compound that embodiment 1 is prepared is measured its structure by the following method; This compound is a yellow jelly, and the compound 1 H and 13 C NMR spectral data (as table 1, figure 1 and figure 2 ) shows that it contains 18 carbons and 16 hydrogens, including one 1,2,4,5-tetrasubstituted benzene ring (C-5~C-10, H-5, H-8), one 1, 4-Disubstituted benzene ring (C-1'~C-6', H-3',5' and H-2',6'), 1 α,β-unsaturated carbonyl (C-2, C -3, C-4, H-2), 1 hydroxypropyl group (C-1"~C-3", H 2 -1”~H 2 -3"), and two phenolic hydroxyl groups (δ H 10.72s and 10.98s). According to the typical signal of two benzene rings and α,β-unsaturated carbonyl, it can be speculated that the compound is an isoflavone compound. According to H-2 and C-3, C-4, C-9, C-1', H-5 and C-4, C-9, C-10, H-8 and C-6, C-7, C-9, C-10, and the HMBC correlation of H-2', 6' and C-2 (such as image 3 ) can further confirm that the compound is an isoflavone structure.

[0043] After the parent of ...

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Abstract

The invention discloses an isoflavone compound extracted from pawpaw, and a preparation method and application thereof. The molecular formula of the isoflavone compound is C18H16O5; the structural formula of the isoflavone compound is as shown in the specification; and the isoflavone compound is named as 6,4'-dihydroxy-7-(3-hydroxypropyl)-isoflavone. The isoflavone compound is prepared from pawpawthrough extraction of extract, column chromatography on silica gel and high-pressure liquid chromatographic separation. Experimental results show that the compound has good antioxidation activity andfree-radical removal activity; and when applied as an anti-oxidant for perfume, the compound can effectively inhibit oxidation deterioration of the perfume and substantially prolong the shelf life ofthe perfume without any influence to the smoking quality of cigarettes.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to an isoflavone compound extracted from papaya for the first time, and the compound has good antioxidant activity. At the same time, the invention also relates to the preparation method of the compound and the application of the compound in inhibiting the oxidative deterioration of spices. Background technique [0002] Papaya (scientific name: Chaenomelessinensis (Thouin) Koehne), is the fruit of the genus Papaya in the family Rosaceae. Oval, 10-15 cm long, dark yellow, woody, sweet and sour, rich in aroma. It has health effects such as appetizing, breast enhancement and beautifying, and treating rheumatism. Papaya can also be used to make preserved fruit, fruit wine, sparkling wine, etc. You can also cook dishes, such as: all kinds of papaya chicken, papaya fish, stewed pig's feet with papaya and so on. It is delicious and is a green fruit with delicious tas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/36C07D311/40A24B15/40
CPCA24B15/403C07D311/36C07D311/40
Inventor 陈章玉向海英凌军高茜张天栋蒋次清王晋许永孔维松周敏杨叶昆李雪梅胡秋芬杨光宇
Owner CHINA TOBACCO YUNNAN IND
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