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Preparation method of gypenoside TN-1

A technology of Gypenosides and Gypenosides, which is applied in the field of Gynostemma pentaphyllum, which can solve the problems of difficult large-scale extraction and low yield, and achieve the effect of improving anti-tumor and promoting in-depth development and utilization

Inactive Publication Date: 2018-04-13
厦门华侨亚热带植物引种园
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  • Abstract
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  • Claims
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Problems solved by technology

At present, there is no literature that reports on the preparation method and biological activity of Gyp TN-1 in detail. It is only found that Gyp TN-1 can be directly extracted from Gynostemma pentaphyllum, but the yield is very low
See the literature "Dammarane-type saponins from Gynostemma pentaphyllum [J]. Phytochemistry, 2011, 72 (11-12): 1453-1459"; researchers such as Kim from the aerial part of Gynostemma pentaphyllum of 1.085kg, leaching by methanol at room temperature for 3 weeks After extraction, use ODS silica gel column chromatography or MCI column chromatography to finally obtain 18 mg of Gyp TN-1, with a yield of only 0.00166%, so it is difficult to directly extract a large amount of Gyp TN-1 from Gynostemma pentaphyllum

Method used

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  • Preparation method of gypenoside TN-1

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Embodiment 1

[0026] The preparation method of embodiment 1 gypenoside TN-1

[0027] A preparation method of Gypenoside TN-1, comprising the following preparation methods:

[0028] Step 1. Extraction of the total saponins of Gynostemma pentaphyllum: dry the aerial part of Gynostemma pentaphyllum at 50°C, pulverize it, add ethanol with a volume fraction of 80% for 3 times, and the ratio of solid to liquid is 1:4 (g / mL). After extracting for 4 hours, the extract was concentrated at 40°C with a rotary evaporator until it was in the form of an extract to obtain the Gynostemma pentaphyllum extract, which was dispersed and suspended in distilled water, then extracted three times with an equal volume of petroleum ether, and the petroleum ether layer was discarded. The layer was extracted with n-butanol for 3 times, the lower layer was discarded, the combined n-butanol layers were concentrated under reduced pressure, concentrated by a rotary evaporator at 45°C, and dried by a freeze dryer to obtain...

Embodiment 2

[0033] The structural identification of embodiment 2 enzymatic conversion products

[0034] The chemical structures of the enzymatic conversion products were identified by NMR methods. Dissolve the sample to be identified in 1 mL of deuterated methanol, seal it and test it. Test items include 1H-NMR, 13 C-NMR, DEPT45, DEPT135, HMBC, HSQC, and 1 H- 1 H COZY, the test results are as follows Figure 1 to Figure 7 shown. The enzymatic conversion product compound is a white amorphous powder, easily soluble in methanol; the carbon spectrum data and hydrogen spectrum data (such as Table 1) of this compound are consistent with the literature report of Gyp TN-1, so it is determined that this compound is: Gyp TN-1 1.

[0035] Table 1 NMR spectrum data of enzymatic conversion products

[0036]

[0037]

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Abstract

The invention discloses a preparation method of gypenoside TN‑1, which comprises the following preparation methods: step 1, extraction of total gypenosides; step 2, separation and purification of gypenoside XLVI; step 3, enzymatic conversion of gypenoside XLVI; step 4. Preparation of Gypenoside TN‑1. The present invention adopts the enzymatic conversion method for the first time, and a large amount of gypenoside TN‑1 can be produced from Gynostemma pentaphyllum, so that gypenoside TN‑1 replaces ginsenoside IH901 to generate significant economic value, thereby improving the antitumor and other medicinal values ​​of Gynostemma pentaphyllum, and promoting the production of Gynostemma pentaphyllum. in-depth development and utilization.

Description

technical field [0001] The invention relates to the technical field of Jiaogulan, in particular to a preparation method of Jiaogulan saponin TN-1. Background technique [0002] Gynostemma pentaphyllum (Thunb.) Makino., also known as Aescin, five-leaf ginseng, seven-leaf ginseng and Ganman tea, etc., is a perennial climbing plant of the genus Gynostemma cucurbitaceae. It is a traditional Chinese herbal medicine commonly used in my country. It is the only non-ginseng plant known to contain ginsenosides. According to statistics, there are currently 17 species and 2 varieties of Gynostemma pentaphyllum in the world, of which 14 species and 2 varieties exist in China. The main distribution areas are Southeast Asia and other places, as well as the Qinling Mountains and the south of the Yangtze River in China. On March 5, 2002, Gynostemma pentaphyllum was included in the list of health products by the Ministry of Health, becoming a new resource for both medicine and food. [0003]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/00
CPCC12P33/00
Inventor 郑志忠明艳林陈良华黄雯郑国华
Owner 厦门华侨亚热带植物引种园
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