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Method for detecting residual erythromycin in bacterial residue

A detection method, the technology of erythromycin, is applied in the detection field of erythromycin residual potency in bacterial residue, which can solve the problems of accurate detection of erythromycin residue in bacterial residue, etc., and achieve the effect of reducing influence and improving accuracy

Active Publication Date: 2018-04-13
NINGXIA HOPE FIELD BIOLOGICAL AGRI TECH CO LTD
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Problems solved by technology

[0005] In view of the current situation that there is no standard detection method for erythromycin residues in bacterial residues at home and abroad, there is no effective solution to the influence of the matrix effect, and the existing technology cannot quickly / accurately detect the problem of erythromycin residues in bacterial residues. Invention and establishment of a detection method for erythromycin residues in bacterial residues

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  • Method for detecting residual erythromycin in bacterial residue
  • Method for detecting residual erythromycin in bacterial residue
  • Method for detecting residual erythromycin in bacterial residue

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specific Embodiment approach 1

[0027] Specific embodiment one: the detection method of erythromycin residue in a kind of bacterial residue of the present embodiment, it is carried out according to the following steps:

[0028] 1. Sample preparation for the test product:

[0029] 1) Weigh the bacterium residue containing erythromycin, add extractant acetonitrile-Tris-CaCl 2 solution, vortexed for 45-60s, then assisted extraction in ultrasound for 20-30min, then centrifuged at 3500-4000rpm for 5-10min, took the supernatant, and collected the precipitate;

[0030] 2) Add extractant acetonitrile-Tris-CaCl to the precipitate collected in step 1) 2 solution, vortexed for 45-60s, then assisted extraction in ultrasound for 20-30min, then centrifuged at 3500-4000rpm for 5-10min, and took the supernatant;

[0031] 3) Combine the supernatants of step 1) and step 2), and put them in a water bath at 35°C for rotary evaporation to one-fifth of the volume of the supernatant to obtain an inorganic phase;

[0032] 4) Add...

specific Embodiment approach 2

[0041] Embodiment 2: The difference between this embodiment and Embodiment 1 is that the pH of the dipotassium hydrogen phosphate solution is 8-10. Others are the same as the specific embodiment one.

specific Embodiment approach 3

[0042] Embodiment 3: This embodiment differs from Embodiment 1 in that: the residual amount of erythromycin in the fungus residue is 500-1000 mg / kg. Others are the same as the specific embodiment one.

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Abstract

The invention discloses a method for detecting residual erythromycin in bacterial residue, and relates to a method for detecting valence of the residual erythromycin in the bacterial residue. The method comprises the steps of preparing a reference solution, preparing a test solution, injecting the reference solution and the test solution into a liquid chromatograph instrument in a gradient elutionmanner through high-performance liquid chromatography, and calculating the valence of erythromycin according to an external standard method. A liquid chromatographic analysis method for the erythromycin in the bacterial residue is created through optimization of chromatographic conditions and tests of preparation methods of a test substance and a reference substance; the method for detecting theerythromycin in the bacterial residue is created through optimization of a chromatographic separation system; and methodology proof tests and researches show that the created detection method has highaccuracy, strong specificity and good reproducibility, and can detect the valence of the erythromycin in the bacterial residue effectively.

Description

technical field [0001] The invention relates to the technical field of detection and inspection of fungus residues, in particular to a method for detecting residual potency of erythromycin in fungal residues. Background technique [0002] Erythromycin (Erythromycin) was discovered by Mc Guire et al. in 1952. It is the main species of fourteen-membered macrolide antibiotics produced by Streptomyces erythraeus. Erythromycin is divided into erythromycin A, B, C and D, among which erythromycin A has antibacterial activity and is the main active ingredient of erythromycin. Although the antibacterial spectrum and antibacterial activity of erythromycin B are similar to those of erythromycin A, erythromycin B is more toxic. Erythromycin C is not an active substance, but is the main impurity of erythromycin produced in my country. Over the years, pharmaceutical workers from all over the world have conducted a series of extensive and in-depth studies on the chemical structure and an...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/74
CPCG01N30/02G01N30/06G01N30/74
Inventor 王义张鹏金昌福李宁飞
Owner NINGXIA HOPE FIELD BIOLOGICAL AGRI TECH CO LTD
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