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A high-efficiency chlorothalonil-degrading bacterium and its application in greenhouse soil environment

A technology of chlorothalonil and degrading bacteria, which is applied in the field of chlorothalonil-efficient degrading bacteria and its application in the greenhouse soil environment, can solve the problems of bioremediation that have not been reported, and achieve the reduction of soil genotoxicity and no secondary pollution , high efficiency effect

Active Publication Date: 2020-06-05
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report about the bioremediation of chlorothalonil-contaminated soil in the greenhouse environment by using micro-acidophilic Stenotrophomonas acidaminiphila in the present invention

Method used

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  • A high-efficiency chlorothalonil-degrading bacterium and its application in greenhouse soil environment
  • A high-efficiency chlorothalonil-degrading bacterium and its application in greenhouse soil environment
  • A high-efficiency chlorothalonil-degrading bacterium and its application in greenhouse soil environment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Growth of bacterial strain BJ1 in inorganic salt medium with chlorothalonil as the only carbon source and its degradation effect on chlorothalonil

[0016] Configure OD 600 The strain mother solution with a value of 1 was inoculated into an inorganic salt medium containing 50 mg / L chlorothalonil as the sole carbon source with an inoculum volume of 5% by volume. The liquid volume was 30 mL, the initial pH value was 6, and the culture temperature The temperature is 30°C, the rotation speed is 180rpm / min, no inoculation is used as the control, and 3 parallels are set for each treatment. After culturing for 1, 3, 5, and 7 days, the content of chlorothalonil in the culture medium was detected by gas chromatography. The results showed that after inoculation with chlorothalonil-degrading bacteria BJ1, the residual amount of chlorothalonil in the medium gradually decreased with time, and the residual amount of chlorothalonil was 3.4 mg / L after 7 days, and the degrada...

Embodiment 2

[0019] Colonization of the soil in the greenhouse environment of embodiment 2 bacterial strain BJ1

[0020] The experiment was carried out in the greenhouse of the Agricultural Demonstration Park of Qingdao Agricultural University. The soil used is taken from the demonstration garden, which is brown loam soil. The fungicide chlorothalonil was not detected in the soil by gas chromatography. The physical and chemical properties are: pH, 6.32; total organic carbon, 17.5g / kg; total nitrogen, 1.35g / kg; total phosphorus, 1.04g / kg. Transfer the soil through a 40-mesh sieve into 3L brown flowerpots, put 1.5kg of soil in each flowerpot, and set up 3 treatments in total: the soil without pesticides and bacteria is the control (CK), and only the pesticides are added. Soil without bacteria (NC), soil with pesticides and bacteria (NCB). Three parallel sets were set up for each treatment, with a total of 9 flowerpots. The initial concentration of chlorothalonil added to the soil was 20m...

Embodiment 3

[0021] Degradation of chlorothalonil in soil by bacterial strain BJ1 under embodiment 3 greenhouse environment

[0022] Soil treatment, concentration of chlorothalonil added, amount of bacteria BJ1 added and its culture conditions were the same as those of NC and NCB in Example 2. Samples were taken 2h, 3, 7, 10, 14, 21, and 28d after treatment, respectively, and the residual amount of chlorothalonil in the soil was detected by gas chromatography. The result is as figure 2 As shown, after the strain was added to the chlorothalonil-contaminated soil, the degradation rate of chlorothalonil was significantly increased, and the degradation half-life was shortened from 9.0d to 4.9d, and the degradation effect was better.

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Abstract

The invention relates to a degrading bacterium BJ1 capable of efficiently degrading the fungicide chlorothalonil and its application in soil in a greenhouse environment, belonging to the technical field of biodegradation, and the degrading bacterium is identified as Stenotrophomonas microacidophilus acidaminiphila), the strain preservation number is CCTCC NO: M2011475. The strain is a degrading bacterium that can use chlorothalonil as the sole carbon source, and the degradation rate of 50 mg / L chlorothalonil can reach 93.2% after 7 days in the inorganic salt culture solution. In the high-temperature and high-humidity greenhouse environment, the application of strain BJ1 can promote the degradation of chlorothalonil in soil, the degradation rate can be increased by 45.6%, and can significantly reduce the soil genotoxicity induced by chlorothalonil. The invention is mainly used for the biological purification of chlorothalonil residues in the soil, especially in the greenhouse environment, reduces the harm caused by the chlorothalonil to the environment, restores the chlorothalonil-contaminated soil, and protects the ecological environment.

Description

technical field [0001] The invention belongs to the technical field of biodegradation, and relates to a chlorothalonil highly efficient degrading bacterium and its application in the soil environment of a greenhouse. Background technique [0002] Chlorothalonil, the chemical name is 2,4,5,6-tetrachloro-1,3-benzenedicarbonitrile, the molecular formula is C 8 C l4 N 2 , the molecular weight is 265.91. As a high-efficiency broad-spectrum chlorinated benzene aromatic agricultural fungicide, chlorothalonil is widely used in vegetables, fruit trees, rice, wheat, cotton and other crops to treat downy mildew, powdery mildew, gray mold, anthracnose, etc. A variety of fungal diseases have good control effect. In my country, the annual output of chlorothalonil is close to 9000t, which is the second most used fungicide. Chlorothalonil is relatively stable in the environment, and its half-life in the farmland soil ecosystem is as long as 6 months. At the same time, due to its wide u...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 张清明王彩霞
Owner QINGDAO AGRI UNIV
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