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Fusogenic liposome-coated porous silicon nanoparticles

A nanoparticle and liposome technology, applied in liposome delivery, nanotechnology, nanotechnology, etc., can solve problems such as low therapeutic efficacy

Active Publication Date: 2018-04-17
RGT UNIV OF CALIFORNIA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Endocytosis is the main uptake route for most nanoplatforms, which causes lysosomal degradation of genetic material and low therapeutic efficacy

Method used

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  • Fusogenic liposome-coated porous silicon nanoparticles
  • Fusogenic liposome-coated porous silicon nanoparticles
  • Fusogenic liposome-coated porous silicon nanoparticles

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0107] Fused Silicon Nanoparticle Synthesis.

[0108] Using a lipid solution of a mixture of DMPC:DSPE-PEG:DOTAP at a molar ratio of 76.2:3.8:20, 21 μL of 1.25 mg / ml (2Z)-2-[(E)-3-(3,3 -Dimethyl-1-octadecylindole-1- -2-yl)prop-2-enyl]-3,3-dimethyl-1-octadecylindole (DiI) to prepare fusogenic liposome membranes.

[0109] Porous silicon nanoparticles (pSiNPs) were prepared by electrochemical etching of single crystal silicon wafers in aqueous HF ethanol, followed by removal of the porous layer and sonication.

[0110] The liposome mixture was dried into a film and hydrated with a solution of porous silicon or calcium silicate deposited porous silicon nanoparticles. The hydrated suspension was heated to 40° C. for 20 minutes under magnetic stirring and mechanically extruded twenty times through a polycarbonate membrane with 200 nm pores.

[0111] Figure 1B -H shows a schematic diagram of particle synthesis and characterization data. Scattering and microscopy data of fused ...

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Abstract

The disclosure describes a fusogenic liposome-coated porous silicon nanoparticles for high loading efficiency of anionic payloads (small molecules, dyes, nucleic acids), and for non-endocytic deliveryof hydrophilic and lipophilic payloads by membrane fusion. The liposome coating can be further modified with targeting peptides or antibodies via covalent binding chemistry between the ligands and functionalized poly (ethylene glycol). The surface moieties can be transferred to the cellular membrane surface by fusogenic uptake. The composition of the disclosure can be applied in the treatment ofdiseases by delivering entrapped / encapsulated payloads.

Description

[0001] Statement Regarding Federally Funded Research [0002] This invention was supported by grant number HR0011-13-2-0017 awarded by the Defense Advanced Research Projects Agency (DARPA) and DMR-1210417 awarded by the National Science Foundation. The government has certain rights in this invention. [0003] Cross References to Related Applications [0004] This application claims priority under 35 U.S.C. § 119 (35 U.S.C. § 119) to Provisional Application Serial No. 62 / 190,705, filed July 9, 2015, the disclosure of which is incorporated by reference This article. technical field [0005] The present invention relates to delivery systems, and more particularly, fused liposomal nanoparticle compositions for the delivery of drugs, nucleic acids, and peptides to target cells or tissues. Background technique [0006] In vivo gene delivery remains a challenge due to low efficiency or cytotoxicity. Endocytosis is the main uptake route for most nanoplatforms, which leads to lys...

Claims

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Application Information

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IPC IPC(8): A61K8/14A61K9/127A61K31/695B82Y5/00B82Y30/00
CPCA61K47/6949A61K47/6923A61K47/6913A61K9/1272A61K47/24A61K31/713A61P27/02A61P35/00A61P31/04B82Y5/00B82Y30/00A61K9/0048A61K9/0051A61K9/1271A61K9/5115A61K47/64
Inventor 迈克尔·J·塞罗尔B·金康珍英
Owner RGT UNIV OF CALIFORNIA
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