Streptococcus pneumoniae protein vaccine and preparation method thereof

A technology for protein vaccines and pneumococci, which is applied in the direction of antibacterial drugs, bacterial antigen components, carrier-bound antigen/hapten components, etc. It can solve the problems of easily blocked absorption, poor immune response, and inability to produce immune memory, so as to improve antibody production. Concentration and seroconversion rate, the effect of reducing the probability of inoculation failure

Inactive Publication Date: 2018-04-20
BRAVOVAX
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0011] Since polysaccharide vaccines only contain polysaccharide antigens (a T cell-independent antigen), they can stimulate mature B lymphocytes, but not T lymphocytes, and cannot produce immune memory
The immune function of infants under 2 years old is not fully developed, and the immune response to polysaccharides is very poo...

Method used

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  • Streptococcus pneumoniae protein vaccine and preparation method thereof
  • Streptococcus pneumoniae protein vaccine and preparation method thereof
  • Streptococcus pneumoniae protein vaccine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] 1. Lectin preparation

[0053]Take 500 grams of fresh pinellia tubers from Shanghai University of Traditional Chinese Medicine, wash and grind, centrifuge at 6000rpm / min for 10min, and the supernatant is fractionally purified by ammonium sulfate with a saturation degree of 20% to 95%, and then collect 60% to 95% saturated sulfuric acid Ammonium precipitates PTA, and the active components are subjected to mannose-Sepharose gel chromatography in stages to obtain Pinellia lectin protein solution for future use. Alternatively, the lectin can be cloned into Escherichia coli for expression and isolated and purified for future use.

[0054] 2. Preparation of pneumococcal capsular saccharide

[0055] 1. Select seven serotypes (4, 6B, 9V, 14, 18C, 19F and 23F) for culture of pneumococci;

[0056] 2. Respectively purify the capsular sugars with strong antigenicity in the above various serotypes of pneumococci: centrifuge to collect the supernatant after inactivation of pneumoco...

Embodiment 2

[0064] The difference between this example and Example 1 is that the lectin is the gore agglutinin detoxified from the agarose beads, and the obtained protein vaccine solution is numbered as sample 2.

Embodiment 3

[0066] The difference between this example and Example 1 is that the serotype and dosage of the vaccine capsular saccharide are 4g each of 1, 4, 5, 7F, 9V, 14, 18C, 19F and 23F, and 8g for 6B.

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Abstract

The invention discloses a Streptococcus pneumoniae protein vaccine, which is a Streptococcus pneumoniae capsule saccharide-protein conjugate obtained by covalently linking one or a variety of Streptococcus pneumoniae capsule saccharides and a carrier protein, wherein the carrier protein is lectin. Compared to the Streptococcus pneumoniae protein vaccine in the prior art, the Streptococcus pneumoniae protein vaccine of the present invention has the following advantages that the administration mode of the Streptococcus pneumoniae protein vaccine is intranasal administration, wherein the intranasal administration is convenient and rapid compared to intramuscular injection; the Streptococcus pneumoniae protein vaccine can be used as the commercially available Streptococcus pneumoniae supplementary immunization vaccine; and when the large-scale epidemic situation occurs, the Streptococcus pneumoniae protein vaccine can be temporarily used as the emergency vaccine, and can provide the immunization foundation for the subsequent vaccination of the commercially available Streptococcus pneumoniae vaccine.

Description

technical field [0001] The invention relates to a pneumococcal protein vaccine, in particular to a pneumococcal protein vaccine which can be used for nasal administration. Background technique [0002] The epidemiology of pneumococcal disease [0003] The high morbidity, high fatality rate, antibiotic resistance and serious sequelae of pneumococcal disease (PDs), especially the serious impact of the disease on infants and the elderly, make this disease an important global disease. One of the public health problems, my country is also a country with a high incidence of pneumococcal disease. Streptococcus pneumoniae (SP, also known as Streptococcus pneumoniae, Pneumococcus) belongs to the genus Streptococcus and is a catalase-negative Gram-positive diplococcus. The capsule of the outer cell wall is a special structure of bacteria. A layer of mucus material outside the cell wall of some bacteria can protect bacteria from antibacterial or bactericidal substances and phagocytosi...

Claims

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Application Information

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IPC IPC(8): A61K39/09A61K39/385A61K39/116A61P31/04
Inventor 艾智武袁军程超张凡吴克
Owner BRAVOVAX
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