Radix aconiti brachypodi polysaccharide, as well as extraction method and application

An extraction method, polysaccharide technology, applied in the field of biomedicine, to achieve good economic and social benefits, simple extraction method, high efficiency

Active Publication Date: 2018-04-20
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Drugs with definite curative effects, such as Bulleaconitine A, have been developed from the genus Aconitum and used in clinical practice; however, the clinical application and some pharmacological effects of Aconitum plants cannot be fully explained by diterpene alkaloid components, and some scholars speculate that other A class of biologically active polysaccharides may also play an important role in the clinical treatment and drug application of Aconitum plants; the study of polysaccharides in Aconitum began in 1986, and Konno et al. reported that they were isolated from the rhizomes of Aconitum japonica Four polysaccharides with hypoglycemic activity
Afterwards, some studies on the extraction, separation, structure identification, content determination, and biological activity of polysaccharides from plants of the genus Aconitum (mainly medicinal Aconitum) were carried out successively; Relevant research reports, therefore, carrying out the extraction method and biological function research of Artemisia chinensis polysaccharide is conducive to fully exploring the medicinal value of Artemisia chinensis

Method used

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  • Radix aconiti brachypodi polysaccharide, as well as extraction method and application
  • Radix aconiti brachypodi polysaccharide, as well as extraction method and application
  • Radix aconiti brachypodi polysaccharide, as well as extraction method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0023] A method for extracting Artemisia spp. polysaccharides, comprising the following steps:

[0024] (1) Rough extraction; get Artemisia spp., reflux and degrease 2 times with 90% ethanol with a mass percentage concentration, filter to obtain filter residue; add water to extract in the filter residue, wherein the quality of adding water is 6% of the quality of the filter residue times; the number of times of water extraction is 3 times, and the time of each water extraction is 30 minutes; the extraction method is carried out by decoction. Combine and concentrate the obtained extracts, add absolute ethanol, mix well, let stand for alcohol precipitation for 24 hours, collect the precipitate, and dry to obtain the crude extract of Artemisia spp.

[0025] (2) Dissolving the crude extract of Artemisia spp. with water, adding Sevag reagent (chloroform:n-butanol=5:1, V / V) to the solution, mixing, and standing to obtain a deproteinized polysaccharide solution; The volume ratio of ...

Embodiment 2

[0028] A method for extracting Artemisia spp. polysaccharides, comprising the following steps:

[0029] (1) Coarse extraction; get Artemisia spp., reflux and degrease 2 times with 95% ethanol with mass percent concentration, filter to obtain filter residue; add water to extract in the filter residue, wherein the quality of adding water is 8% of the quality of the filter residue times; the number of times of water extraction is 5 times, and the time of each water extraction is 20 minutes; the extraction method is carried out by decoction. Combine and concentrate the obtained extracts, add absolute ethanol, mix well, let stand for alcohol precipitation for 36 hours, collect the precipitate, and dry to obtain the crude extract of Artemisia chinensis;

[0030] (2) Dissolving the crude extract of Artemisia spp. with water, adding Sevag reagent (chloroform:n-butanol=5:1, V / V) to the solution, mixing, and standing to obtain a deproteinized polysaccharide solution; The volume ratio o...

Embodiment 3

[0033] A method for extracting Artemisia spp. polysaccharides, comprising the following steps:

[0034] (1) Coarse extraction; Get Artemisia spp., reflux and degrease 2 times with 70% ethanol with a mass percentage concentration, filter to obtain filter residue; add water to extract in the filter residue, wherein the quality of adding water is 4% of the quality of the filter residue times; the number of times of water extraction is 1 time, and the time of water extraction is 150 minutes; the extraction method is carried out by decoction. Combine and concentrate the obtained extracts, add absolute ethanol, mix evenly, let stand for alcohol precipitation for 12 hours, collect the precipitate, and dry to obtain the crude extract of Artemisia spp.

[0035] (2) Dissolving the crude extract of Artemisia spp. with water, adding Sevag reagent (chloroform:n-butanol=5:1, V / V) to the solution, mixing, and standing to obtain a deproteinized polysaccharide solution; The volume ratio of so...

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Abstract

The invention discloses an extraction method of radix aconiti brachypodi polysaccharide. The extraction method comprises the following steps: (1) coarse extraction: performing reflux degreasing on radix aconiti brachypodi with ethanol, adding water for extraction, concentrating obtained extracting solution, adding anhydrous ethanol into the extracting solution, uniformly mixing the mixture, allowing standing still for ethanol precipitation, collecting precipitations and drying, so as to obtain radix aconiti brachypodi crude extract; (2) dissolving the radix aconiti brachypodi crude extract with water, adding an Sevag reagent into dissolved solution, mixing the mixture and standing, so as to obtain deproteinized polysaccharide solution; (3) refining: loading a polysaccharide solution sampleon a gel column or macroporous adsorption resin, eluting the polysaccharide solution sample with an eluent, collecting eluent and drying, so as to obtain the radix aconiti brachypodi polysaccharide.The extraction method has the advantages that the extraction method is simple and is high in efficiency, the polysaccharide can be extracted from the radix aconiti brachypodi, the radix aconiti brachypodi polysaccharide can be enabled to have an effect on preparation of cytokines for promoting proliferation of splenic lymphocytes, and the new medicinal value of the radix aconiti brachypodi is developed, so that the extraction method has good economic and social benefits.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to polysaccharides from Artemisia persicae and its extraction method and application. Background technique [0002] Aconitum brachypodum (Aconitum brachypodum) is the dry tuberous root of Aconitum brachypodum, a plant of the genus Aconitum in the Ranunculaceae family. It is mainly distributed in the northeast and northwest of Yunnan and southwest of Sichuan, and is wild in high mountains at an altitude of 3100-4100 meters. Meadows, rocky hillsides. It is warm in nature, bitter in taste, pungent, highly toxic, and has the functions of expelling wind and dampness, benefiting joints, reducing swelling and relieving pain. The genus Aconitum plays an important role in traditional medicine in my country. At present, more than 40 species of Aconitum plants have been used as medicine; Aconite and other 6 kinds. [0003] The chemical constituents in Artemisia japonica are m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P37/04
CPCA61K31/715C08B37/0003
Inventor 左爱学万春平张建英郑喜
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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