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Electrochemical detection method for uric acid in urine and detection electrode material

A technology for detecting electrodes and uric acid electrodes, which is applied in the field of detection, can solve problems such as differences in electrode detection performance, low detection limit, and limited use range, and achieve good detection stability and small relative standard deviation.

Inactive Publication Date: 2018-04-20
THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These detection methods have their own advantages and disadvantages. Chromatography is the most basic detection method, with good separation effect, simple mobile phase, easy and fast operation, but requires multi-step sample processing; spectroscopic method is susceptible to other chromophores in the sample. Although the enzymatic method has good selectivity, it is expensive, which limits its scope of application; the electrochemical method has the characteristics of simple operation, low cost, high sensitivity, and low detection limit.
[0004] However, there is a problem with the electrochemical method: the prepared electrodes are greatly affected by the preparation parameters, and it is easy to have large differences in the detection performance of the electrodes in the batch, which affects the detection accuracy.

Method used

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  • Electrochemical detection method for uric acid in urine and detection electrode material
  • Electrochemical detection method for uric acid in urine and detection electrode material

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Uric acid electrochemical detection electrode material steps:

[0036] Step 1, preparing graphene oxide by the Hummers method;

[0037] The second step, the reduction of graphene oxide: disperse the graphene oxide obtained in the first step in water to obtain a 4 mg / mL graphene oxide dispersion, take 50 mL of the graphene oxide dispersion, and slowly drop 30 μL of 70wt % hydrazine hydrate solution, then reacted at 85°C for 1h, filtered out the product, washed, and dried to obtain graphene;

[0038] The 3rd step, the acid modification of graphene: by weight, mix 12 parts of graphene with 25 parts of acid mixed solution, then heat reflux reaction 2h under ultrasonic action, described acid mixed solution refers to by volume A mixture of 75wt% concentrated sulfuric acid and 50wt% concentrated nitric acid compounded at a ratio of 3:1. After the reaction, the product was filtered out, washed with deionized water, and dried to obtain acid-modified carbon nanotubes;

[0039] ...

Embodiment 2

[0045] Uric acid electrochemical detection electrode material steps:

[0046] Step 1, prepare graphene oxide by Hummers method;

[0047] The second step, the reduction of graphene oxide: disperse the graphene oxide obtained in the first step in water to obtain a 8 mg / mL graphene oxide dispersion, take 60 mL of the graphene oxide dispersion, and slowly drop 50 μL of 80wt % hydrazine hydrate solution, then reacted at 90°C for 3h, filtered out the product, washed, and dried to obtain graphene;

[0048] The 3rd step, the acid modification of graphene: by weight, mix 20 parts of graphene with 30 parts of acid mixed solution, then heat and reflux reaction 4h under ultrasonic action, described acid mixed solution refers to by volume A mixture of 85wt% concentrated sulfuric acid and 60wt% concentrated nitric acid compounded at a ratio of 4.5:1. After the reaction is completed, the product is filtered out, washed with deionized water, and dried to obtain acid-modified carbon nanotubes...

Embodiment 3

[0055] Uric acid electrochemical detection electrode material steps:

[0056] Step 1, prepare graphene oxide by Hummers method;

[0057] The second step, the reduction of graphene oxide: disperse the graphene oxide obtained in the first step in water to obtain a 5mg / mL graphene oxide dispersion, take 55mL of the graphene oxide dispersion, and slowly drop 40μL of 75wt % hydrazine hydrate solution, then reacted at 88°C for 2h, filtered out the product, washed, and dried to obtain graphene;

[0058] The 3rd step, the acid modification of graphene: by weight, mix 15 parts of graphene and 28 parts of acid mixed solution, then heat reflux reaction 3h under ultrasonic action, described acid mixed solution refers to by volume A mixture of 80wt% concentrated sulfuric acid and 55wt% concentrated nitric acid compounded at a ratio of 4:1. After the reaction, the product was filtered out, washed with deionized water, and dried to obtain acid-modified carbon nanotubes;

[0059] Step 4, ac...

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Abstract

The invention relates to an electrochemical detection method for uric acid in urine and a detection electrode material, and belongs to the technical field of detection. The electrochemical detection method for uric acid in urine provided by the invention is mainly used for conducting hydroxyl modification on the surface of graphene, cyanide-containing groups can be grafted more easily, and then acatalyst is deposited on the surface in an electrolytic deposition manner, so that the electrode material with preferable catalytic effect on uric acid is prepared; and when the material is applied todetection in urine, the material has preferable detection stability for uric acid, and the relative standard deviation among the detection results of electrodes prepared in batches is relatively low.

Description

technical field [0001] The invention relates to an electrochemical detection method of uric acid in urine and a detection electrode material, belonging to the technical field of detection. Background technique [0002] High uric acid is likely to cause gout, so prevention of gout is the first priority. Generally speaking, gout is a group of diseases that cause tissue damage due to the increase of blood uric acid due to the disorder of purine metabolism. Lesions often invade tissues such as joints and kidneys. The age of onset is mostly above 40, and the prevalence rate increases with age. The ratio of male to female is 50:1. Most gynecological patients are postmenopausal women, and the disease often occurs in spring and autumn. Therefore, special attention should be paid to patients with high blood uric acid. [0003] At present, the detection and analysis methods of uric acid include fluorescence method, high performance liquid chromatography, enzymatic method and elect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/48
Inventor 于世金
Owner THE AFFILIATED HOSPITAL OF QINGDAO UNIV
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