Plasma fibrinogen detection reagent, plasma fibrinogen detection method, and applications of plasma fibrinogen detection reagent

A fibrinogen and detection reagent technology, applied in the field of clinical medical detection, can solve the problems such as the repeatability and stability of the kit need to be improved, and achieve the effects of accurate detection, simple operation and simple reagents

Inactive Publication Date: 2018-04-20
北京众驰伟业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] CN 101221184A discloses a kind of in-vitro diagnostic kit that uses the Clauss method to check plasma fibrinogen content, and this kit includ

Method used

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  • Plasma fibrinogen detection reagent, plasma fibrinogen detection method, and applications of plasma fibrinogen detection reagent

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Embodiment 1

[0057] Embodiment 1: the detection reagent of preparation plasma fibrinogen

[0058] The preparation method of the detection reagent of described plasma fibrinogen comprises the steps:

[0059] (1) Prepare plasma diluent R1: mix imidazole with a mass concentration of 3.8%, 0.48% NaCl and polyethylene glycol 0.12% with an average molecular weight of 8000, adjust the pH to 7.2, and make up the remainder with water to obtain a plasma diluent R1;

[0060] (2) preparation thrombin buffer system R2: the mass concentration is 3.8% imidazole, 0.4% BSA, 0.08% Triton-X-100, 4% mannitol, 4% glycine, 1.5% trehalose and 200IU / mL thrombin was mixed and adjusted to pH 7.2, and finally 0.05% proclin-300 was added, and the balance was made up with water to obtain thrombin buffer system R2;

[0061] The R1 and R2 are plasma fibrinogen reagents prepared in the present invention.

Embodiment 2

[0062] Embodiment 2: the detection reagent of preparation plasma fibrinogen

[0063] The preparation method of the detection reagent of described plasma fibrinogen comprises the steps:

[0064] (1) Prepare plasma diluent R1: mix imidazole with a mass concentration of 1%, 0.1% NaCl and polyethylene glycol with an average molecular weight of 15,000 0.01%, adjust the pH to 7.2, and make up the remainder with water to obtain a plasma diluent R1;

[0065] (2) Preparation of thrombin buffer system R2: the mass concentration of 1% imidazole, 0.1% BSA, 0.01% Triton-X-100, 1% mannitol, 1% glycine, 1% trehalose and Mix 50IU / mL thrombin and adjust the pH to 7.2, finally add 0.01% proclin-300, and make up the rest with water to obtain the thrombin buffer system R2;

[0066] The R1 and R2 are plasma fibrinogen reagents prepared in the present invention.

Embodiment 3

[0067] Embodiment 3: the detection reagent of preparation plasma fibrinogen

[0068] The preparation method of the detection reagent of described plasma fibrinogen comprises the steps:

[0069] (1) Preparation of plasma diluent R1: mix imidazole with a mass concentration of 15%, 5% NaCl and polyethylene glycol 1% with an average molecular weight of 5000, adjust the pH to 7.2, and make up the remainder with water to obtain a plasma diluent R1;

[0070] (2) preparation thrombin buffer system R2: the mass concentration is 15% imidazole, 10% BSA, 1% Triton-X-100, 15% mannitol, 15% glycine, 10% trehalose and 400IU / mL thrombin was mixed and adjusted to pH 7.2, and finally 1% proclin-300 was added, and the rest was made up with water to obtain thrombin buffer system R2;

[0071] The R1 and R2 are plasma fibrinogen reagents prepared in the present invention.

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Abstract

The invention belongs to a clinical medicine detection technology, and particularly relates to a plasma fibrinogen detection reagent, a plasma fibrinogen detection method, and applications of the plasma fibrinogen detection reagent, wherein the plasma fibrinogen detection reagent comprises a plasma diluent R1 and a thrombin buffer system R2, the plasma diluent R1 comprises imidazole, NaCl and polyethylene glycol, and the thrombin buffer system R2 comprises imidazole, Triton-X 100, mannitol, glycine, trehalose, bovine serum albumin, Proclin-300 and thrombin. According to the present invention,the detection method has advantages of high precision and simple operation; and the safe, reliable and good-repeatability in vitro diagnostic reagent is provided for the clinical detection of plasma fibrinogen.

Description

technical field [0001] The invention belongs to clinical medical detection technology, and in particular relates to a detection reagent for plasma fibrinogen, a detection method and an application thereof. Background technique [0002] Coagulation test is of great significance to the diagnosis of various clinical diseases. In addition to the screening and diagnosis of bleeding diseases, it is also used for the examination of prethrombotic state; the experimental diagnosis of disseminated intravascular coagulation bleeding disease (DIC) Medication guidance and prognosis estimation for anticoagulants. At present, there are four coagulation tests (including prothrombin time PT, activated partial thromboplastin time APTT, thrombin time TT, and fibrinogen FIB content) commonly used clinically to detect the coagulation mechanism. [0003] Thrombosis and hemostasis not only involve basic medicine, but are also closely related to diseases in multiple clinical disciplines (including...

Claims

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Application Information

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IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/75
Inventor 杨军京
Owner 北京众驰伟业科技发展有限公司
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