Liver-blood brain barrier system for simulating in-vivo metabolic process based on micro-fluidic chip

Abstract

The invention provides a liver-blood brain barrier system for simulating the in-vivo metabolic process based on a micro-fluidic chip. The micro-fluidic chip is mainly composed of a top chip, a porousfilter membrane and a bottom chip, wherein liver cells are inoculated to the top chip, so that the liver metabolic process of the medicine in the body can be simulated; the bottom chip is mainly formed in a manner that a left main channel and a right main channel are connected through a collagen channel, and a brain colloid cell layer and a brain microvascular endothelial cell layer are inoculatedto a collagen interface, so that the blood brain barrier function can be simulated. According to the system, the construction and representation of an in-vitro blood brain barrier model are integrated with the evaluation for the permeability of the liver metabolism process of the medicine on a barrier, and the system can be applied to in-vitro simulation of the blood brain barrier model and the medicine evaluation. With the system provided by the invention, the problems of secondary inoculation and long consumed time of a cell co-culture model are solved, the flow conditions are added, so that the real environment in the body is well conformed, meanwhile, the consumption amount of cells and reagents is obviously reduced, and a plurality of experimental relevant parameters can be simultaneously obtained at one time.

Description

technical field [0001] The invention relates to the application of microfluidic chip technology to the field of simulation and application of in vivo tissue engineering, in particular to a microfluidic chip-based liver-blood-brain barrier system for simulating metabolic processes in vivo. Background technique [0002] Animal experiments occupy an extremely important position in modern medicine and biology, but funding and animal ethics have also become unavoidable issues. Combining microfluidic technology and bioscience technology, an "organ chip" has been created, which can replicate the functions of human organs with microchips, making medical experiments easier. [0003] After drugs enter the body, they often go through processes such as absorption, distribution, metabolism, and excretion. The drug enters the blood circulation through the shielding membrane composed of cells from the outside or the site of administration, and enters the liver through the portal vein. Und...

AI Technical Summary

Problems solved by technology

[0005] Since the development of cell biology, the existing in vitro blood-brain barrier simulation methods mainly focus on commercialized orifice plates or Transwell chambers to study the structure and function of the two-dimensional blood-brain barrier model. The main problem is that the orifice plate or Transwell It is difficult for the small chamber to construct a blood-brain barrier model in vitro with interface conversion from two-dimensional plane to three-dimensional space, and it is difficult to realize in situ observation of mesenchymal stem cells crossing the blood-brain barrier for intracerebral tumor treatment

[0008] At present, the use of microfluidic technology to construct functional complex multi-organ chips for related research and analysis is still in a blank stage. If it can be realized, it will have great application prospects in biological research and pharmaceutical research and development.

Images