Liver-Brain Barrier System Based on Microfluidic Chip to Simulate Metabolic Processes in vivo

Abstract

A liver-blood-brain barrier system based on a microfluidic chip for simulating metabolic processes in vivo. The microfluidic chip is mainly composed of a top chip, a porous filter membrane, and a bottom chip. The top chip is inoculated with liver cells, which can simulate the liver metabolism process of drugs in vivo; the bottom chip is mainly connected by the left main channel and the right main channel through the collagen channel, and the brain glial cell layer and the brain microvascular endothelial cell layer are seeded on the collagen interface Can simulate blood-brain barrier function. The invention integrates the construction and characterization of the in vitro blood-brain barrier model and the evaluation of the barrier permeability of the drug through the liver metabolism process, and can be used for the in vitro simulation of the blood-brain barrier model and the application of drug evaluation. The invention solves the problem of secondary inoculation and long time consumption of the cell co-culture model, adds flow conditions, is more in line with the real environment in the body, and significantly reduces the consumption of cells and reagents, and can obtain multiple experimental related parameters at the same time.

Description

technical field [0001] The invention relates to the application of microfluidic chip technology to the field of simulation and application of in vivo tissue engineering, in particular to a microfluidic chip-based liver-blood-brain barrier system for simulating metabolic processes in vivo. Background technique [0002] Animal experiments occupy an extremely important position in modern medicine and biology, but funding and animal ethics have also become unavoidable issues. Combining microfluidic technology and bioscience technology, an "organ chip" has been created, which can replicate the functions of human organs with microchips, making medical experiments easier. [0003] After drugs enter the body, they often go through processes such as absorption, distribution, metabolism, and excretion. The drug enters the blood circulation through the shielding membrane composed of cells from the outside or the site of administration, and enters the liver through the portal vein. Und...

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Problems solved by technology

[0005] Since the development of cell biology, the existing in vitro blood-brain barrier simulation methods mainly focus on commercialized orifice plates or Transwell chambers to study the structure and function of the two-dimensional blood-brain barrier model. The main problem is that the orifice plate or Transwell It is difficult for the small chamber to construct a blood-brain barrier model in vitro with interface conversion from two-dimensional plane to three-dimensional space, and it is difficult to realize in situ observation of mesenchymal stem cells crossing the blood-brain barrier for intracerebral tumor treatment

[0008] At present, the use of microfluidic technology to construct functional complex multi-organ chips for related research and analysis is still in a blank stage. If it can be realized, it will have great application prospects in biological research and pharmaceutical research and development.

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