Culture medium for fagus longipetiolata plant tissue culture and culture method thereof

Abstract

The invention belongs to the technical field of plant tissue culture and discloses a culture medium for fagus longipetiolata plant tissue culture and a culture method thereof. The material drawing period is April; a culture medium for starting culture is as follows: leaf, MS+2,4-D2.0mg / L+6-BA1.0mg / L+saccharose25g / L, and stem, MS+2,4-D1.0mg / L+6-BA1.5mg / L+saccharose30g / L; a sugar source with the effect of promoting callus tissue differentiation is MS+6-BA3.0mg / L+NAA0.5mg / L+saccharose20g / L; and a culture medium for rooting is 1 / 2MS+NAA1.5mg / L+saccharose30g / L. The culture medium capable of cultivating fagus longipetiolata tissue culture seedlings within short time is screened out, and the reliability is high.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and in particular relates to a culture medium and a culture method for tissue culture of plants of the genus Aquacarpus. Background technique [0002] At present, my country's Shuiqinggang wood is widely used in decorative panels, veneers, floors, wall panels, plywood, furniture and building materials, and is also a good oil tree species. In terms of forestry production, on the one hand, soil erosion and environmental pollution are becoming increasingly serious, leading to forest depletion; on the other hand, human-made excessive logging of forest resources has caused a sharp decrease in the number of plants of the genus Aquacarpus. Most of the wood of the genus Aquacarpus is precious wood. Due to historical reasons and traditional production habits, at present, the original products of the genus Aquacarpus are in short supply in the international and domestic markets. In addition to...

AI Technical Summary

Problems solved by technology

[0003] In summary, the existing problems in the prior art are: because the plants of the genus Cyclops have higher economic value, they are widely developed and utilized at present, resulting in poor resources, which are in short supply in the market; the germination rate of seeds is low, and the natural germination rate is not enough 3%, manual treatment, the germination rate is only 30%; the genetic differentiation of seed propagation is large, the excellent traits of the mother plant cannot be maintained, and the seedling growth consistency is poor

Images