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Method for extracting and preparing polysaccharose from callicarpa nudiflora

A technology for violet purpurea and violacea polysaccharide is applied in the field of extraction and preparation of polysaccharides, which can solve the problems of large dosage, poor controllability of quality standards, complex chemical composition of traditional Chinese medicines, etc., and achieves stable quality, convenient operation and good accuracy. Effect

Inactive Publication Date: 2018-05-01
海南制药厂有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are disadvantages such as large doses of traditional Chinese medicine, unclear active ingredients, and poor controllability of quality standards, which are related to the complex chemical composition of traditional Chinese medicine and the unclear mechanism of drug action.

Method used

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  • Method for extracting and preparing polysaccharose from callicarpa nudiflora
  • Method for extracting and preparing polysaccharose from callicarpa nudiflora
  • Method for extracting and preparing polysaccharose from callicarpa nudiflora

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Embodiment 1

[0038] A method for extracting and preparing polysaccharides from Auranthus nudica, comprising:

[0039] Preparation steps: take fresh Auranthus nudiflora leaves, wash them with clean water, dry them in a cool place, remove rotten leaves and insect spots, and cut 50g of dried Aurora nudiflora leaves to 1-5mm with scissors to obtain Fragments of naked flower purple beads;

[0040] One extraction step: add the fragments of Aurantia nudica to 95% ethanol whose mass is 10-20 times that of the leaves of Aurantia nudica, soak at room temperature for 4-6 hours, filter out the ethanol solution, and soak in 95% ethanol in the same way Extract 3-4 times, combine the ethanol extracts to recover ethanol; pour out the 95% ethanol-extracted fragments of the nudibranch on a clean table, dry the residual ethanol on the fragments of the nudibranch, and obtain the primary extract;

[0041]Secondary extraction step: add the primary extract to water with a mass 10-20 times that of the leaves of ...

Embodiment 2

[0045] A method for extracting and preparing polysaccharides from Auranthus nudica, comprising:

[0046] Preparation steps: take fresh Auranthus nudiflora leaves, wash them with clean water, dry them in a cool place, remove rotten leaves and insect spots, and crush 250g of dried Aurora nudiflora leaves to 0.1-5mm with a pulverizer to obtain Fragments of naked flower purple beads;

[0047] One extraction step: add the fragments of Aurantia nudica to 95% ethanol whose mass is 10-20 times that of the leaves of Aurantia nudica, soak at room temperature for 4-6 hours, filter out the ethanol solution, and soak in 95% ethanol in the same way Extract 3-4 times, combine the alcohol extracts to recover ethanol; pour out the ethanol-extracted nudibranch fragments onto a clean table, dry the residual ethanol on the nudibranch fragments, and obtain a primary extract;

[0048] Secondary extraction step: add the primary extract to water with a mass 10-20 times that of the leaves of Auranthu...

Embodiment 3

[0052] Compared with Embodiment 2, this embodiment differs in that, referring to Figure 1-3 , after the refining step is completed, the detection and analysis step is also included: using high performance liquid exclusion chromatography combined with a CAD detector, using dextran as a standard solution, and using the Chromeleon 7Extension Pack GPC Templates software of ThermoFisher Ultra3000 to map and analyze, the naked The number average molecular weight of Huazizhu polysaccharide is 16.79×10 4 , with a weight average molecular weight of 74.7×10 4 .

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Abstract

The invention discloses a method for separating and preparing water-soluble polysaccharose of callicarpa nudiflora. The callicarpa nudiflora leaves are subjected to percolation extraction for many times by ethanol; then, callicarpa nudiflora crude polysaccharide is obtained through methods of water boiling extraction, alcohol precipitation and alcohol repeated washing; finally, semipermeable membrane dialysis and gel filtering are performed to obtain refined callicarpa nudiflora polysaccharose with higher purity. Through primary qualitative and quantitative analysis, the molecular weight and the monosaccharide composition of the callicarpa nudiflora polysaccharose are determined. The water-soluble polysaccharose ingredients in the callicarpa nudiflora can be separated, so that the qualityof the callicarpa nudiflora can be well controlled; the method can be used for evaluating the authenticity and the quality of callicarpa nudiflora medicinal materials; the quality stability, uniformity and controllability are ensured.

Description

technical field [0001] The invention relates to a method for separating and purifying natural products, in particular to a method for extracting and preparing polysaccharides from the medicinal plant Aurora nudiflora. Background technique [0002] Callicarpa nudiflora Hook.et Arn. is a plant of the genus Callicarpa nudiflora (Verbenaceae). It is mainly produced in Hainan, my country. It is an authentic medicinal material in Hainan, and it is also distributed in Guangdong and Guangxi. Foreign countries are mainly distributed in India, Vietnam, Singapore and Malaysia. Its branches and leaves can be used as medicine. It has the functions of antibacterial and hemostasis, anti-inflammatory and detoxification, dispelling stasis and swelling, and dispelling wind and dampness. , burns and traumatic bleeding. [0003] According to literature research reports, the main chemical components of Nadiflora medicinal materials include flavonoids, tannins, terpenoids, and volatile oils, am...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 蒲含林赖潜李秀峰
Owner 海南制药厂有限公司
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