ATPSb gene related to survival of brown planthopper and encoded protein as well as application thereof

Abstract

The invention discloses a ATPSb gene related to survival of a brown planthopper and encoded protein as well as application thereof. The ATPSb gene is characterized in that the ATPSb gene has nucleotide sequence shown as SEQ ID NO: 1, a gene code ATP synthase b subunit, plays an important role in keeping normal survival of the brown planthopper, which cause the decreasing of the survival rate if the role is inhibited. The protein encoded by the ATPSb gene has the amino sequence shown as SEQ ID NO: 2. The application of the ATPSb gene and encoded protein is used for researching pesticide and biologically controlling the brown planthopper. According to the invention, the RNA interference on the ATPSb gene is successfully conducted, the results show that the survival rate of pests is reduced,the survival of the pests is obviously inhabited, so that the functions that the pests are inhabited and the biologically controlling is fully realized are hopefully realized at the same time.

Description

technical field [0001] The invention relates to a brown planthopper survival-related gene, encoded protein and application thereof. Background technique [0002] Brown planthopper ( Nilaparvata lugens Stål) is a monophagous rice pest with long-distance migratory characteristics and strong reproductive ability. Brown planthoppers mainly damage rice by feeding on rice phloem juice, causing delayed growth, delayed tillering, increased empty grains, and in severe cases, the whole plant dies. In addition, brown planthoppers can also transmit a variety of rice viruses. At present, the emergency control of brown planthoppers is mainly based on chemical control, but chemical control is likely to lead to the increase of pest resistance, the residue of harmful substances on rice, the rampant pests and environmental pollution. Among the pesticides used to control brown planthoppers, the effective pesticides that have played a good role in the past, such as fipronil, imidacloprid, b...

AI Technical Summary

Problems solved by technology

At present, the emergency control of brown planthoppers is mainly based on chemical control, but chemical control is likely to lead to the increase of pest resistance, the residue of harmful substances on rice, the rampant pests and environmental pollution.

Among the pesticides used to control brown planthoppers, the effective pesticides that have played a good role in the past, such as fipronil, imidacloprid, buprofezin, etc., have been eliminated or restricted in use. Feel helpless in the choice of pesticides when carrying out control

Related genes such as detoxification enzymes and metabolic enzymes that have been identified so far cannot explain the mechanism of pest resistance well, and there is still a lack of sustainable and effective control methods for brown planthopper disasters

The reason is that most of these chemical pesticides aim at large-scale and high-intensity killing of pests. However, due to practical reasons such as the extremely complex genetic diversity of the brown planthopper population, some individuals often survive, and as a result of high selection pressure, eventually lead to the formation of more adaptive populations

On the other hand, while chemical pesticides kill pests, they will also endanger non-target organisms including natural enemies, which will inevitably have a negative impact on the farmland ecosystem, resulting in the failure of the ecological control function to be fully exerted.

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