The method of SNP typing by direct PCR of oral swab
An oral and swab technology, applied in the field of SNP typing, can solve the problems of expense, high cost, and a lot of time
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0050] A method for performing SNP typing by direct PCR of oral swabs, comprising the following steps: rough processing of oral swabs, obtaining diluted samples—PCR amplification—SNP typing identification; strong tolerance is used in the PCR amplification. Sexual DNA polymerase.
[0051] The rough treatment method of the buccal swab is to add 200 μl of cell lysate to the buccal swab storage tube and then lyse at room temperature for 10 minutes; The supernatant was put into a 96-well plate; the dilution refers to diluting the cell solution 5 times with distilled water to obtain a diluted sample. Beginning with lysis, followed by removal of epidermal cells by centrifugation, and finally by 5-fold dilution, a sample that can be used for PCR was obtained through these operations. During this process, we did not use any additional reagents or instruments, and completed it in the simplest way. This pretreatment process is the first of its kind in this application.
[0052] The str...
Embodiment 2
[0069] A method for performing SNP typing by direct PCR of oral swabs, comprising the following steps: rough processing of oral swabs, obtaining diluted samples—PCR amplification—SNP typing identification; strong tolerance is used in the PCR amplification. Sexual DNA polymerase.
[0070] The rough treatment method of the buccal swab is to add 200 μl of cell lysate to the buccal swab storage tube and then lyse at room temperature for 10 minutes; The supernatant was put into a 96-well plate; the dilution refers to diluting the cell solution 5 times with distilled water to obtain a diluted sample. Beginning with lysis, followed by removal of epidermal cells by centrifugation, and finally by 5-fold dilution, a sample that can be used for PCR was obtained through these operations. During this process, we did not use any additional reagents or instruments, and completed it in the simplest way. This pretreatment process is the first of its kind in this application.
[0071] The str...
Embodiment 3
[0088] A method for performing SNP typing by direct PCR of oral swabs, comprising the following steps: rough processing of oral swabs, obtaining diluted samples—PCR amplification—SNP typing identification; strong tolerance is used in the PCR amplification. Sexual DNA polymerase.
[0089] The rough treatment method of the buccal swab is to add 200 μl of cell lysate to the buccal swab storage tube and then lyse at room temperature for 10 minutes; The supernatant was put into a 96-well plate; the dilution refers to diluting the cell solution 5 times with distilled water to obtain a diluted sample. Beginning with lysis, followed by removal of epidermal cells by centrifugation, and finally by 5-fold dilution, a sample that can be used for PCR was obtained through these operations. During this process, we did not use any additional reagents or instruments, and completed it in the simplest way. This pretreatment process is the first of its kind in this application.
[0090] The str...
PUM
Login to View More Abstract
Description
Claims
Application Information
Login to View More