Liquid-phase chip detection system and method based on quantum dots

Abstract

The invention discloses a liquid-phase chip detection system and method based on quantum dots. The liquid-phase chip detection system comprises a liquid-phase chip, a sheath flow formation module, a fluorescence excitation light source, an optical signal detection device and a circuit module, wherein the liquid-phase chip is a quantum dot encoding fluorescent microsphere subjected to chemical modification; the sheath flow formation module comprises a microfluidic channel; the liquid-phase chip is covered with sheath fluid and a laminar flow state is formed in a flowing chamber; then the liquid-phase chip passes through the microfluidic channel in sequence in a single-row straight line and the optical signal detection device is used for detecting to obtain a fluorescent signal; the circuitmodule is composed of a microprocessor and a signal processing circuit. The system disclosed by the invention has the advantages of simple and easy-to-realize structure and high fluorescent detectionaccuracy; interference, caused by the fact that emitted light is overlapped, on a detection result is avoided; the system has the characteristics of high accuracy, high sensitivity and low cost and has a wide market application prospect.

Description

technical field [0001] The invention relates to the field of biomolecular structure detection, in particular to a liquid phase chip detection system and method based on quantum dots. Background technique [0002] Liquid phase chip technology uses microspheres as the reaction carrier to detect proteins, nucleic acids and other biological macromolecules in a liquid reaction system close to the internal environment of the biological system. It can detect multiple indicators at one time and complete the purpose of multiple detection. [0003] At present, the commercialized liquid phase chip detection system on the market is based on the xMAP technology of Luminex Company of the United States, and polystyrene microspheres with a diameter of about 5.6um are mixed with two different organic fluorescent dyes, red and infrared, in a certain proportion. Two laser beams are used to detect the coded fluorescence and reporter fluorescence of the microspheres at the same time. The red las...

AI Technical Summary

Problems solved by technology

[0009] Aiming at the problems of the current technology, the object of the present invention is to provide a liquid phase chip detection system and method based on quantum dots, aiming at solving the problem that the overlapping emitted light will affect the accuracy of the detection results when multiple rows of microspheres pass through the detection area. And the use of CMOS or CCD imaging equipment to detect technical problems that cause high hardware requirements and complex algorithms that are difficult to implement

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