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Rootstock bud configuration method of prunus salicina crown grafting

A configuration method and hollow plum technology are applied in the directions of biochemical equipment and methods, grafting, microbial determination/inspection, etc., which can solve the problems of immature development of sand hollow plum seeds, difficulty in obtaining plum rootstock seedlings, etc., and improve grafting survival. rate effect

Active Publication Date: 2018-05-08
DALIAN NATIONALITIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, in many areas, especially in Yanhe County, plums are rarely used as rootstocks for grafting, and the sand hollow plum seeds in Yanhe County are often immature, so it is difficult to obtain plum rootstock seedlings

Method used

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  • Rootstock bud configuration method of prunus salicina crown grafting
  • Rootstock bud configuration method of prunus salicina crown grafting
  • Rootstock bud configuration method of prunus salicina crown grafting

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1 Extracting Hollow Plum Leaf Genomic DNA

[0020] Taking 21 plum germplasms M1, M2, M3, M4, M6, M7, M8, YH1, YH2, YH3, YH4, YH5, YH6, YH7, YH8, YH9, YH10, YH11, YH30, YH31 in Yanhe County , YH32 and a rootstock seedling ZM5 as materials. According to the method recommended by Tiangen New Plant Genomic DNA Extraction Kit (DP320), the genomic DNA of leaves of different plum germplasms and rootstock seedlings was extracted, and the initial scion of the rootstock seedlings came from the same mother tree. The concentration of genomic DNA of each quality is 20-40ng / μL, and stored at -20°C.

Embodiment 2

[0021] Example 2 Developing Hollow Li SSR Markers

[0022] Using the leaves and fruits of Prunus hollowus as materials, the total RNA was extracted and purified according to the method recommended by the column type plant total RNA extraction and purification kit of Shanghai Sangong Company. RNA-Seq technology was used for high-throughput sequencing of transcriptomes, SSR sites were obtained and primers were designed, 71 pairs of primers were selected, and PCR amplification was performed using 4 different germplasm DNAs of Prunus hollandii as templates. The reaction system was as follows:

[0023]

[0024] The reaction program was: pre-denaturation at 94°C for 4 min; denaturation at 94°C for 30 s, annealing at 58°C for 30 s, extension at 72°C for 30 s, 35 cycles; extension at 72°C for 10 min; storage at 4°C. The PCR results of 71 pairs of SSR primers were detected by agarose gel electrophoresis, and 42 pairs of SSR-labeled primers suitable for hollow plums were screened, as...

Embodiment 3

[0030] Embodiment 3 Select the scion with good fruit quality and close relationship with the rootstock seedling, and design the hollow plum grafting combination

[0031] According to the genetic distance between various germplasms obtained in Example 2 and the rootstock seedling ZM5 and the fruit quality traits of the other 21 plum germplasms, as shown in Table 2 below, a high-grained scion was selected. Select the current-year shoots of germplasm with high sugar-acid ratio (M1, YH4, YH8, YH9) and average single fruit size (YH30, YH31, YH32) as scions, and cut them smoothly from top to bottom to a length of 2 cm, with a little fullness of xylem Buds; select 3-4 year old branches from the rootstock seedlings, and cut them into a smooth cut surface of 2-2.5cm from top to bottom. close. Design 15 plants with the same age and growth potential for each grafting combination, graft 5 buds per plant, and investigate the survival rate after 60 days of grafting, as shown in Table 3 bel...

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Abstract

The invention relates to a rootstock bud configuration method of prunus salicina crown grafting, and belongs to the technical field of plant grafting cultivation. According to the main technical scheme, the method comprises the following steps that genome DNAs of leaves of prunus salicina and rootstock seedlings different in germplasm are extracted separately, wherein initial scions of the rootstock seedlings come from the same mother tree; PCR amplification is carried out on the genome DNAs of the leaves of the prunus salicina and the rootstock seedlings by utilizing a polymorphic prunus salicina SSR marker primer; according to a PCR amplified band result, genetic distances between the prunus salicina and the rootstock seedlings different in germplasm are calculated; the prunus salicina with the genetic distance being 0-0.0081 and good fruit quality and characters is selected, and a grafting combination is designed; prunus salicina crown grafting is carried out by adopting a xylem budgrafting method. The method aims at root tillery seedlings and cutting seedlings low in yield and poor in fruit quality, and by utilizing the between-germplasm molecular genetic distance recognitiontechnology and the xylem grafting method, good-quality germplasm crown grafting of prunus salicina is achieved.

Description

technical field [0001] The invention relates to the technical field of plant grafting and cultivation, in particular to a method for arranging stock buds for grafting and replacing crowns of hollow plum trees. Background technique [0002] Prunus is a perennial tree belonging to the genus Prunus L. of the Rosaceae subfamily Prunoideae. Sand Hollow Plum, named for the natural splitting of the core and pulp when ripe, has been cultivated for more than 130 years. Li Zhong Maotai" reputation. In 2006, it was recognized as a "National Geographical Indication Protection Product" by the State Administration of Quality and Technology Supervision and Administration. In recent years, the use of root tiller seedlings and grafted seedlings of non-improved varieties to build orchards has resulted in a decline in fruit quality (fruit size, taste, peel thickness, etc.) year by year. The main reason is the lack of fine varieties of hollow plum and insufficient supply of seedlings of good...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G2/30A01G2/35C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 丁健阮成江胡国钧吴茂宏张绍阳关莹
Owner DALIAN NATIONALITIES UNIVERSITY
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