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Method for extracting phenylethanoid glycosides substances from cistanche tubulosa and application thereof

A technology of Cistanche pipiensis and phenylethanoid glycosides is applied in chemical instruments and methods, sugar derivatives, sugar derivatives, etc., and can solve the problems of long time consumption, large solvent consumption, low extraction rate and the like, and achieves simple shearing equipment, The effect of less solvent consumption and high extraction efficiency

Active Publication Date: 2018-05-08
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the solvent extraction method has the disadvantages of large amount of solvent and long time consumption.
Microwave extraction has the advantages of simple equipment, short operation time, and less solvent consumption, but its extraction rate is low, it is not easy to automate, and it lacks the possibility of online connection with other instruments
Ultrasonic extraction is easy to operate, simple in equipment, and high in extraction rate, but it has long working hours, consumes a large amount of solvent, and is not easy to automate

Method used

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  • Method for extracting phenylethanoid glycosides substances from cistanche tubulosa and application thereof
  • Method for extracting phenylethanoid glycosides substances from cistanche tubulosa and application thereof
  • Method for extracting phenylethanoid glycosides substances from cistanche tubulosa and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Cistanche tuberosum (purchased from Xinjiang Hetian Cistanche Tang Biotechnology Co., Ltd.) was crushed, passed through a 50-mesh sieve, dried in an oven at 70°C for 12 hours, weighed 14g, and added 126ml of 50% ethanol aqueous solution (solid-liquid ratio 1 :9 (mass g / volume ml)), at 70°C, adjust the speed of the Fluko high-shear homogeneous emulsifier to 16000rpm for extraction, extract once, centrifugally filter, get 1mL filtrate and use Waters high-performance liquid chromatography to detect, the remaining The filtrate was dried under reduced pressure to obtain the phenylethanol glycoside extract which was vacuum freeze-dried into powder. Calculate the total yield of echinacoside and verbascoside in the extract ≥ 2.23%.

Embodiment 2

[0030] Cistanche tuberosum (purchased from Xinjiang Hetian Cistanche Tang Biotechnology Co., Ltd.) was crushed, passed through a 50-mesh sieve, dried in an oven at 70°C for 12 hours, weighed 14g, and added 126ml of 50% ethanol aqueous solution (solid-liquid ratio 1 : 9 (mass g / volume ml)), at 70°C, adjust the speed of the Fluko high-shear homogeneous emulsifier to 19000rpm for extraction, extract once, centrifugally filter, get 1mL filtrate and use Waters high-performance liquid chromatography to detect, the remaining The filtrate was dried under reduced pressure to obtain the phenylethanol glycoside extract which was vacuum freeze-dried into powder. Calculate the total yield of echinacoside and verbascoside in the extract ≥ 2.28%.

Embodiment 3

[0032] Cistanche tuberosum (purchased from Xinjiang Hetian Cistanche Tang Biotechnology Co., Ltd.) was crushed, passed through a 50-mesh sieve, dried in an oven at 70°C for 12 hours, weighed 14g, and added 126ml of 50% ethanol aqueous solution (solid-liquid ratio 1 :9 (mass g / volume ml)), at 70°C, adjust the speed of the Fluko high-shear homogeneous emulsifier to 22000rpm for extraction, extract once, centrifugally filter, get 1mL filtrate and use Waters high-performance liquid chromatography to detect, the remaining The filtrate was dried under reduced pressure and freeze-dried in vacuo to obtain a powdered phenylethanol glycoside extract. Calculate the total yield of echinacoside and verbascoside in the extract ≥ 2.35%.

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Abstract

The invention discloses a method for extracting phenylethanoid glycoside substances from cistanche tubulosa and application thereof. The method for extracting the phenylethanoid glycoside substances from the cistanche tubulosa includes the following steps: step 1, performing crushing: crushing dry succulent stems of the cistanche tubulosa, performing sieving, and performing drying; step 2, performing extraction: adding a 50% ethanol aqueous solution into the cistanche tubulosa powder obtained in the step 1, performing heating to the temperature of 70 DEG C, and performing extraction for 2min by using a high shear homogenizing emulsifying machine at the temperature of 70 DEG C, wherein the extraction is performed once; and step 3, performing centrifugation filtration on the extraction liquid obtained in the step 2, performing reduced-pressure drying, and performing vacuum lyophilization to a powder shape, so as to obtain the phenylethanoid glycoside substances. The method provided by the invention has the advantages of short extraction time, a less solvent usage amount and high extraction efficiency, the high-shear equipment is simple and easy to use in series with other equipment,and the method is convenient for realizing automated production.

Description

technical field [0001] The invention relates to the technical field of compound extraction, in particular to a method for extracting phenylethanol glycosides from Cistanche tubulosa and its application. Background technique [0002] Cistanche tubulosa is a parasitic plant of the family Danaceae, which is produced in Xinjiang, my country, and is listed as top grade in "Shen Ben Cao Jing". The phenylethanol glycosides are the main active ingredients of Cistanche tubulosa. Studies have shown that phenylethanol glycosides have the functions of protecting the liver, anti-oxidation, anti-aging, regulating nerve cells, enhancing immunity, and improving memory. Echinacoside and verbascoside are the most effective components in the phenylethanol glycosides of Cistanche tubulosa, accounting for more than 90% of the total phenylethanol glycosides. Existing methods for extracting phenylethanol glycosides from Cistanche tubulosa include solvent extraction, microwave-assisted extraction...

Claims

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Application Information

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IPC IPC(8): C07H15/18C07H1/08
CPCC07H1/08C07H15/18
Inventor 李雪琴裴文静郭瑞丽张金利
Owner SHIHEZI UNIVERSITY
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