Method used for measuring capillary electrophoresis electroosmotic flow

A capillary electrophoresis and electroosmotic flow technology, which is applied in the field of analytical chemistry, can solve the problems of long consumption time, difficulty in measuring weak electroosmotic flow, and low measurement accuracy, and achieves the effects of saving time, high accuracy and improving measurement accuracy.

Inactive Publication Date: 2018-05-08
BEIJING INSTITUTE OF TECHNOLOGYGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, this method is very difficult for the measurement of wea

Method used

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  • Method used for measuring capillary electrophoresis electroosmotic flow
  • Method used for measuring capillary electrophoresis electroosmotic flow
  • Method used for measuring capillary electrophoresis electroosmotic flow

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0059] image 3 and Figure 4 Under the same conditions, the migration time diagrams using the traditional method and the method for measuring capillary electrophoresis electroosmotic flow according to the embodiment of the present invention are respectively shown. Among them, separation voltage: 10kV / -10kV; separation pressure: 70mbar; detection wavelength: 214nm; pressure during injection: 50mbar for 3 seconds; temperature: 25°C; buffer: phosphate buffer solution with pH7.

[0060] Both the traditional method and the method provided in the examples of the present invention use the same sample concentration, operating system and sample injection conditions. The neutral marker used is DMSO, the concentration is 1% (volume ratio), the operating system is phosphate buffer solution with pH=7, the total length of the capillary is 60 cm, the effective length is 50 cm, and the inner diameter is 75 μm.

[0061] The operation of the traditional method is as follows: the sample is in...

Embodiment 2

[0078] During the electrophoresis analysis process, keep other conditions unchanged, only change the separation pressure, analyze under the separation pressure conditions of 50mbar, 60mbar, 70mbar, 80mbar, and 90mbar respectively, and do five sets of parallel experiments under each separation pressure condition. To explore whether different separation pressures have an effect on the performance of measuring electroosmotic flow. Examples 2 and 1 use the same experimental conditions, including the same capillary length, capillary inner diameter, operating system and samples.

[0079] Process according to the steps described in Example 1, and analyze at separation pressures of 50mbar, 60mbar, 70mbar, 80mbar, and 90mbar respectively. The results obtained are shown in Table 2. Figure 5 shown.

[0080] Table 2

[0081]

[0082]

[0083] It can be concluded from Table 2 that the change of the set value of the separation pressure basically has no effect on the accuracy of th...

Embodiment 3

[0086] During the electrophoresis analysis process, keep other conditions unchanged, only change the voltage, respectively in the voltage conditions of 8kV / -8kV, 10kV / -10kV, 12kV / -12kV, 15kV / -15kV, 18kV / -18kV, 20kV / -20kV Under each voltage condition, three sets of parallel experiments were done. It was investigated whether different analytical voltages had an effect on the performance of measuring electroosmotic flow. Example 3 and Example 1 use the same experimental conditions, the same capillary length, inner diameter, operating system and samples.

[0087] The experimental results are shown in Table 3. Figure 6 shown.

[0088] table 3

[0089]

[0090]

[0091] It can be concluded from Table 3 that the change of the voltage setting value basically has no effect on the stability and accuracy of the electroosmotic mobility measured by the method of the embodiment of the present invention, and there is no certain correlation between the two. From Figure 6 It can b...

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Abstract

The invention belongs to the field of analytical chemistry, and discloses a method used for measuring capillary electrophoresis electroosmotic flow. The method comprises following steps: a buffer containing a dissolved marker is introduced into a capillary tube as a sample; the two sides of the capillary tube are applied with a first voltage and a certain separation pressure 1, and a first migration time of the marker is detected; the sample in introduced into the capillary tube for a second time; the two sides of the capillary tube are applied with a second voltage, and a separation pressure2 equal to the separation pressure 1, and a second migration time is detected, wherein the direction of the separation pressure 1 and the direction of the separation pressure 2 are the same, the voltage amplitude of the first voltage is equal to that of the second voltage, and the polarity of the first voltage is opposite to that of the second voltage; and at last electroosmotic flow mobility is calculated based on the first migration time and the second migration time. The method is capable of increasing electroosmotic flow measuring precision, and realizing rapid measurement of weak electroosmotic flow of coated capillary tubes.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for measuring capillary electrophoresis electroosmotic flow. Background technique [0002] Capillary electrophoresis refers to the efficient and rapid separation of components due to the difference in mobility under the action of a high-voltage direct current electric field, with electro-osmotic flow (EOF) as the driving force and fused silica capillary as the separation channel. New liquid phase separation technology. The reason for the electroosmotic flow is related to the material of the fused silica capillary, the SiO of the fused silica capillary material 2 It can react with strong alkali to form silicate, and its isoelectric point is about 1.5. Therefore, in the commonly used separation buffer solution (pH is generally greater than 2), the silicate on the inner wall of the tube is hydrolyzed into Si-OH and releases H + , so that the inner wall of the fused sil...

Claims

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Application Information

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IPC IPC(8): G01N27/447
CPCG01N27/447
Inventor 徐伟贺木易张文静
Owner BEIJING INSTITUTE OF TECHNOLOGYGY
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