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Method for extracting deep sea fish oil

A technology of deep-sea fish oil and deep-sea fish, which is applied in the direction of fat oil/fat production, fat oil/fat refining, fat production, etc. It can solve the problems of low content and content requirements in the pharmaceutical field.

Inactive Publication Date: 2018-05-15
深圳市娅安科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the total content of DPA and DHA in deep-sea fish oil is only about 21wt.%.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A method for refining deep-sea fish oil, specifically comprising the following steps:

[0026] (1) Preparation of fish oil crude extract:

[0027] Use wild salmon as the raw material, clean the wild salmon and put it into a blender to grind it, then take out the minced meat and cook it with low heat for 40 minutes, cool to 35°C after cooking, then add 1mol / L disodium hydrogen phosphate to adjust the pH to 7.8 , add protease to carry out enzymolysis to obtain the enzymolysis solution, centrifuge the enzymolysis solution at 3800r / min for 10min, separate the upper layer oil to obtain the crude fish oil extract;

[0028] (2) Saponification

[0029] Add 1kg of fish oil crude extract to a water bath stirrer, the temperature of the water bath is 50°C, add 1.6kg of 10mol / L sodium hydroxide solution to the fish oil crude extract while stirring, then add 100g of 20% ethanol solution, and stir 45min for saponification, after standing for 2h, the saponified product is obtained; ...

Embodiment 2

[0033] A method for refining deep-sea fish oil, specifically comprising the following steps:

[0034] (1) Preparation of fish oil crude extract:

[0035] Using artificial salmon as the raw material, clean the artificial salmon and put it into a grinder to grind it, then take out the minced meat and cook it with low heat for 60 minutes, cool to 40°C after cooking, then add 1mol / L disodium hydrogen phosphate to adjust the pH to 8.2 , add protease to carry out enzymolysis to obtain the enzymolysis solution, centrifuge the enzymolysis solution at 4200r / min for 15min, separate the upper layer oil to obtain the crude fish oil extract;

[0036] (2) Saponification

[0037] Add 1kg of fish oil crude extract to a water bath stirrer, the temperature of the water bath is 65°C, add 2kg of 10mol / L sodium hydroxide solution to the fish oil crude extract while stirring, then add 250g of 35% ethanol solution, and stir for 60min Carry out saponification, and obtain the saponified product afte...

Embodiment 3

[0041] A method for refining deep-sea fish oil, specifically comprising the following steps:

[0042] (1) Preparation of fish oil crude extract:

[0043] As raw material for sardines, clean the sardines and put them in a blender to crush them, then take out the minced meat and cook them with low heat for 58 minutes, cool to 43°C after cooking, then add 1mol / L disodium hydrogen phosphate to adjust the pH to 8.0, add protease Carry out enzymatic hydrolysis to obtain the enzymatic hydrolysis solution, centrifuge the enzymatic hydrolysis solution at 4000r / min for 12min, separate the upper layer oil to obtain the crude fish oil extract;

[0044] (2) Saponification

[0045] Add 1kg of fish oil crude extract to a water bath stirrer, the temperature of the water bath is 62°C, add 1.8kg of 10mol / L sodium hydroxide solution to the fish oil crude extract while stirring, then add 200g of 30% ethanol solution, and stir 55min for saponification, after standing for 2.5h, the saponified pro...

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PUM

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Abstract

The invention discloses a method for extracting deep sea fish oil. The method comprises the following steps: fully extracting fish oil in deep-sea fish fat by adopting an enzymolysis method; adoptinga saponification-urea inclusion method, saponifying the crude extract of the fish oil to remove a small amount of saturated fatty acids, adding urea after saponification for further purification, separating out a stable crystal inclusion compound formed from the urea, the saturated fatty acids and monounsaturated fatty acids, washing and drying the extract, thereby obtaining the deep sea fish oilcontaining 80% of EPA and DHA.

Description

technical field [0001] The invention belongs to the field of biological extraction, and in particular relates to a method for extracting deep-sea fish oil. Background technique [0002] Deep-sea fish oil refers to the unsaturated fat extracted from deep-sea fish animals. DPA and DHA rich in deep-sea fish oil are omega-3 polyunsaturated fatty acids that play an important role in human physiological health. These nutrients It can effectively promote the growth and development of the human brain and nervous system, reduce the occurrence of cardiovascular and cerebrovascular diseases, and enhance human immunity. It also has anti-cancer and anti-cancer effects. Health care products made of deep-sea fish oil have been on the market for many years at home and abroad. As people pay more and more attention to omega-3 polyunsaturated fatty acids, deep-sea fish oil, as an important source of pure natural omega-3 polyunsaturated fatty acids, has a promising application prospect. It wil...

Claims

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Application Information

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IPC IPC(8): C11B1/00C11B1/02C11B3/00
CPCC11B1/00C11B1/025C11B3/001
Inventor 段华勇苏茂
Owner 深圳市娅安科技有限公司
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