Reagent and method for identifying FMDV and SVA, and application

A technology of reagents and kits, which is applied in the field of detection and identification of foot-and-mouth disease virus and porcine A-type Seneca virus, can solve problems such as improper prevention and control measures, and achieve the effects of ensuring production safety, reducing economic losses, and strong specificity

Inactive Publication Date: 2018-05-15
SHENZHEN AUDAQUE DATA TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Since SVA is a newly discovered zoonotic disease, the clinical symptoms of infected pigs mainly include vesicular lesions such as blisters and festering wounds on the nose and crown of the

Method used

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  • Reagent and method for identifying FMDV and SVA, and application
  • Reagent and method for identifying FMDV and SVA, and application
  • Reagent and method for identifying FMDV and SVA, and application

Examples

Experimental program
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Embodiment

[0032] 1. Materials and methods

[0033] 1. Test nucleic acid

[0034] Foot-and-mouth disease virus (FMDV), porcine Seneca virus type A (SVA), pseudorabies virus (PRV), swine fever virus (CSFV), swine vesicular disease virus (SVDV), Indiana type vesicular stomatitis virus used in this example (VSV-IN) and New Jersey-type vesicular stomatitis virus (VSV-NJ) and other inactivated strains were obtained by the Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry-Exit Inspection and Quarantine Bureau (hereinafter referred to as "Shenzhen Inspection and Quarantine Bureau Animal and Plant Center") Provide and preserve.

[0035] 2. Main reagents and instruments

[0036]Viral RNA extraction kit MagMAXTM-96 Viral RNA Isolation Kit (AM1836) is a product of Thermo Company in the United States. AgPath-ID TM One-Step RT-PCR kit is a product of ABI company.

[0037] Thermo automatic magnetic bead extraction and purification system, BioDrop ultra-micro nucleic ...

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Abstract

The invention discloses a reagent and method for identifying FMDV and SVA and application. The reagent comprises an FMDV primer probe group and an SVA primer probe group; an upstream primer and a downstream primer of the FMDV primer probe group are respectively sequences shown by Seq ID No. 1 and Seq ID No. 2, and the probe is a sequence shown by Seq ID No. 3 or a reverse complementary sequence; and an upstream primer and a downstream primer of the SVA primer probe are respectively sequences shown by Seq ID No. 4 and Seq ID No. 5, and the probe is the Seq ID No. 6 or the reverse complementarysequence. By adopting the reagent of the invention, the FMDV and the SVA can be simultaneously detected and identified, the specificity is high, the sensitivity is high, and a high-efficient technicalmeans is provided for identifying and detecting foot and mouth disease viruses and swine type-A Seneca viruses. The reagent of the invention is suitable for rapidly identifying and detecting two diseases.

Description

technical field [0001] The application relates to the field of detection and identification of foot-and-mouth disease virus and swine type A Seneca virus, in particular to a reagent, method and application for identification of FMDV and SVA. Background technique [0002] Foot-and-mouth disease (FMD for short) is an acute, febrile, contagious animal infection caused by foot-and-mouth disease virus (FMDV) that infects cloven-hoofed animals such as pigs, cattle, and sheep. Epidemic, the infected animals develop high fever, the clinical symptoms are mainly characterized by blisters and erosions in the oral mucosa, nipple skin and hoof crown skin, and hemorrhagic gastroenteritis and myocarditis in young animals. The World Organization for Animal Health (OIE) lists foot-and-mouth disease as a legally notifiable disease, and my country lists it as a first-class animal disease. [0003] Porcine Seneca virus A (abbreviated as SVA) and FMDV and porcine vesicular disease virus (abbrev...

Claims

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Application Information

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IPC IPC(8): C12Q1/70C12Q1/686C12N15/11C12R1/93
CPCC12Q1/686C12Q1/701C12Q2600/16C12Q2537/143C12Q2563/107
Inventor 花群义林彦星花群俊曹琛福杨俊兴黄超华曾少灵阮周曦张彩虹林庆燕
Owner SHENZHEN AUDAQUE DATA TECH
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