Method for accurately measuring true pH (potential of hydrogen) values of intestinal environments of insects

Abstract

The invention discloses a method for accurately measuring true pH (potential of hydrogen) values of intestinal environments of insects. The method includes the steps: firstly, acquiring intestinal tracts of the insects, collecting a certain quantity of intestinal tracts of the insects and contents of the intestinal tracts, and grinding the intestinal tracts of the insects and the contents of the intestinal tracts by a biological tissue grinder; secondly, performing centrifugation by a refrigerated centrifuge to obtain supernate of the intestinal tracts of the insects and the contents of the intestinal tracts, rapidly measuring the pH values of the supernate by wide pH test paper and precision pH test paper to obtain the true pH values of the intestinal environments of the insects. The truepH values of the intestinal environments of some insect larvae can be accurately acquired within a short time, subsequent research of intestinal microorganisms and intestinal enzyme systems of the insects can be facilitated, and the method has the advantages of simplicity in operation and true and reliable measured values. Besides, the method has important guidance values for food digestion, nutrition acquisition and research of biological pesticides related to intestines of the insects.

Description

technical field [0001] The invention belongs to the insect intestinal environment measurement technology, in particular to a method for accurately measuring the real pH value of the insect intestinal environment. Background technique [0002] The digestive tract of insects mainly has the functions of digesting food particles, absorbing nutrients, maintaining ion balance in the body and excreting metabolic waste. According to different functions, it is divided into foregut, midgut and hindgut. Among them, the foregut has a crop for temporary storage of food, and the midgut is the place where intestinal commensal organisms, digestive enzymes and proteases mainly exist, and their functions are most prominent. The midgut mainly secretes digestive juices, digests food and absorbs nutrients. The main function of the hindgut (including the ileum and rectum) is to eliminate nitrogenous metabolic wastes and food residues while providing a favorable nutritional environment for insec...

AI Technical Summary

Problems solved by technology

In this method, the midgut and its contents were eluted with 2 mL of ultrapure water in a beaker and then the pH value was measured, because the original H ion concentration in the intestinal fluid was reduced by adding ultrapure water to the original intestinal fluid. Diluted, so the measured value does not reflect the true pH of the intestinal tract

Articles published by Wang Shenzhu and others: "Identification of the Main Protease Activity in the Midgut of Cotton Bollworm Larvae", (Acta Entomology, Vol. The effect of protease activity in the midgut of the larvae of the pear borer larvae" (Acta Entomology, 2016, Volume 59, Issue 10, 1069-1078), the methods in this paper are: by setting multiple buffers with different concentration gradients in the pH range of 5.5-11.0 The enzyme activity is measured separately in the liquid, and the pH with the highest enzyme activity is determined as the optimum pH value; this method not only requires a lot of work, but also the measured results cannot reflect the enzyme activity under the real intestinal pH value.