Method to survey petroleum contamination distribution by multidimensional gene quantitative technique based on mathematical statistics
A technology of mathematical statistics and pollution distribution, which is applied in the measurement/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems that HoB cannot be cultivated, it is difficult to reflect all HoB information in situ, and it is difficult to quantify data, so as to broaden the scope of application , both reliability and portability
Inactive Publication Date: 2018-05-18
INST OF HYDROGEOLOGY & ENVIRONMENTAL GEOLOGY CHINESE ACAD OF GEOLOGICAL SCI
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In the past, the traditional culture counting method was used for HoB detection. However, most HoB cannot be cultured, and it is difficult to reflect all HoB information in situ. Strictly speaking, this method can only be used qualitatively, and it is difficult to quantify the data.
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[0059] This embodiment includes the following seven specific steps: sample point layout and collection, sample oil, gas and petroleum indicator microorganism gene quantitative test, data accumulation frequency curve drawing, segmental optimal curve regression analysis, petroleum indicator microorganism partition determination, petroleum pollution Plane distribution description, composition characteristics of petroleum pollutants, and delineation of pollution source areas.
[0060] It includes the following steps:
[0061] (1) Arrangement and collection of sample points
[0062] For the site to be investigated, according to the requirements of mathematical statistics, the surface soil sampling points are mainly arranged with grid points. For the key areas suspected of oil pollution, the sample points are densely arranged, and the distance is within 30m. There is no distance limit for boundary control points to meet the control requirements. The purpose is to collect soil sampl...
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The invention discloses a method to survey petroleum contamination distribution by a multidimensional gene quantitative technique based on mathematical statistics and belongs to the field of petroleumcontamination site surveying. The method is characterized by comprising: sampling a site to be surveyed, extracting DNA from a sample, quantifying hydrocarbon oxidizing bacteria via RT-PCR (real-timefluorescent quantitative polymerase chain reaction), and using quantitative data to analyze petroleum contamination distribution characteristics. Various functional genes and underwater traditional contamination indicator CODcr (chemical oxygen demand) are measured, the mathematical statistics method is introduced, the advantages of quantitative multidimensional data are given to full play, and the method to survey petroleum contamination distribution by the multidimensional gene quantitative technique based on mathematical statistics is formed; the gene quantitative technique is verified bycomparison with CODcr; the method enables underground petroleum contamination plane distribution to be quickly and effectively recognized, contaminant compositional characteristics are depicted, and acontamination source range is delineated.
Description
technical field [0001] The invention relates to a method for investigating the distribution of petroleum pollution by a multi-dimensional gene quantitative technology based on mathematical statistics. It belongs to the field of oil pollution site investigation. Background technique [0002] Petroleum pollution has a wide range, great harm, and long cycle. Pollution investigation is the basis for the assessment and restoration of contaminated sites. Among the many physical and chemical drilling survey techniques, the soil gas survey technique (Soil-gas surveying techniques) started in the 1980s is a rapid, economical and effective geochemical prospecting technique for surveying the scope of pollution. Based on the characteristics of volatile organic compounds (VOC) in petroleum pollutants migrating to the surface, this technology predicts the distribution range of underground petroleum pollution by detecting VOC in topsoil. At present, soil VOC detection mainly includes in-...
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IPC IPC(8): C12Q1/6851C12Q1/64
CPCC12Q1/64C12Q1/6851C12Q2561/113C12Q2563/107C12Q2531/113
Inventor 张敏何泽宁卓郭彩娟陈宗宇
Owner INST OF HYDROGEOLOGY & ENVIRONMENTAL GEOLOGY CHINESE ACAD OF GEOLOGICAL SCI