Kit for quantitatively detecting GFAP (glial fibrillary acidic protein) through magnetic microparticle chemiluminescence and preparation method of kit

A quantitative detection, magnetic particle technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, measurement devices, etc., can solve the problems of low resolution sensitivity, affecting the sensitivity of quantitative detection, etc. Detection limit and sensitivity, increase the effect of effective collision

Inactive Publication Date: 2018-05-18
SOPHONIX CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the quantitative detection of this invention is based on the color strength of the reaction between the enzyme HRP and the substrate, and the resolution sensitivity through color difference is low, which affects the sensitivity of quantitative detection.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] The kit includes: magnetic separation reagents, antibody reagents, enzyme-labeled reagents, substrates, washing solutions, calibration products, quality control products, magnetic sleeves and suction heads;

[0048] The magnetic separation reagent is a Tris buffer bottle containing streptavidin-labeled magnetic particles and bovine serum albumin;

[0049] The antibody reagent is a Tris buffer bottle containing biotin-labeled antibody and bovine serum albumin;

[0050] The enzyme-labeled reagent is a Tris buffer bottle containing alkaline phosphatase-labeled antibody and bovine serum albumin;

[0051] The substrate is a Tris buffer bottle containing lucigenin;

[0052] The washing solution is a Tris buffer bottle containing Tween-20 and NaCl;

[0053] The calibrator includes 2 liquid calibration bottles, and the target concentration in the liquid calibration bottle is respectively 0.2ng / ml, 25ng / ml GFAP antigen and the Tris buffer of bovine serum albumin;

[0054] Des...

Embodiment 2

[0059] The preparation method of kit as described in embodiment 1, comprises the steps:

[0060] Step 1: Prepare Buffer A solution

[0061] Prepare 1000ml Buffer A solution, accurately weigh 1.5601g NaH 2 PO 4 2H 2 O, add in pure water, stir until completely dissolved and set the volume to 1000ml, measure the pH value of the solution with pH test paper to determine whether the pH value is between 5.9-6.1, if the pH value exceeds 5.9-6.1, use 0.04M NaOH Or HCl to adjust to within the pH value range, if the concentration is too high to adjust the pH is not accurate, if the concentration is too low, the amount of liquid that needs to be added dropwise is too large, resulting in a larger volume and affecting the concentration of the solute. Then use a 0.22 μm filter membrane to filter the solution. filter;

[0062] Step 2: Prepare Buffer B solution

[0063] Prepare 1000ml Buffer B solution, accurately weigh 0.0499g NaH2PO4 2H2O and 0.6023gNa 2 HPO 4 12H 2 O, add in pure wa...

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Abstract

The invention relates to the field of medical in vitro diagnosis, in particular to a kit for quantitatively detecting GFAP (glial fibrillary acidic protein) through magnetic microparticle chemiluminescence and a preparation method of the kit. The kit for quantitatively detecting the GFAP through the magnetic microparticle chemiluminescence comprises a magnetic separation reagent, an antibody reagent, an enzyme labeled reagent, a substrate, washing liquid, a calibration product, a quality control product, a magnetic sleeve and a sucker. The invention also discloses the preparation method of thekit. By adopting the kit provided by the invention, the detection upper limit and the reaction speed can be increased, and the sensitivity of a detection result is improved.

Description

technical field [0001] The invention relates to the field of medical in vitro diagnosis, in particular to a kit for quantitative detection of GFAP by magnetic particle chemiluminescence and a preparation method thereof. Background technique [0002] Glial fibrillary acidic protein (GFAP) is a specific acidic protein in the cytoplasm of astrocytes (AS) in the central nervous system. It is considered to be a unique marker of AS, and it plays a role in maintaining the morphology and function of AS. In recent years, studies have found that serum and cerebrospinal fluid GFAP levels have important guiding significance in the diagnosis, treatment and prognosis of neurological diseases. For example, serum GFAP is elevated in traumatic brain injury (TBI), which is of great significance for the early diagnosis, differential diagnosis and prognosis of TBI; the plasma GFAP level of patients with acute ischemic stroke is significantly higher than that of healthy controls, and is associat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/536G01N33/543G01N21/76
CPCG01N33/536G01N21/763G01N33/54326
Inventor 杨涛黎霞高丹丹
Owner SOPHONIX CO LTD
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