Micro-imaging system with combined optical tweezers function and expanded focal depth

Abstract

The invention discloses a micro-imaging system with a combined optical tweezers function and expanded focal depth. The micro-imaging system comprises a first laser, a first reflector, a first spectroscope, a first lens, a second lens, a first acousto-optic deflector, a third lens, a fourth lens, a fifth lens, a second acousto-optic deflector, a sixth lens, a second spectroscope, a first dichroic mirror, a second dichroic mirror, a first object lens, a sample table, a second object lens, a third dichroic mirror, a third spectroscope, a fourth spectroscope, a fifth spectroscope, a first four-quadrant position detector, a second four-quadrant position detector, a position detector, an LED light source, a first detector, a second laser, a first cylindrical lens, a cylindrical lens group, a fourth dichroic mirror, a scanning galvanometer, a first telescope system, an electric control lens, a second telescope system, a second reflector, a third telescope system, a slit, a third reflector, asecond cylindrical lens, and a second detector. The system utilizes an electric control lens to scan planes with different focal depths and has the advantages of no mechanical vibration and high imaging quality.

Description

technical field [0001] The invention relates to the fields of microscopic imaging and optical tweezers, in particular to an extended focal depth microscopic imaging system combined with the function of optical tweezers. Background technique [0002] In the study of cell biology, it is often necessary to simultaneously study the mechanics and mechanical properties of biomolecules in cells and the three-dimensional structure of cells. Therefore, in the actual research process, the single-molecule mechanics microscopy system combined with the optical tweezers module and the fluorescence microscopy imaging module is usually used for experiments. When using the single-molecule mechanical microscopy system to operate the sample, the optical tweezers module can realize the measurement of the dynamic characteristics of single molecules in biological cells, and the fluorescence microscopy imaging module can obtain biological structures, extracellular or intracellular, etc. multi-dim...

AI Technical Summary

Problems solved by technology

[0004] Aiming at the limitation of the scanning depth in the sample depth direction existing in the real-time three-dimensional imaging of the biological structure of the sample by the existing single-molecule mechanical microscopy system, the present invention proposes an extended focal depth microscopic imaging system combined with the function of optical tweezers

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