Special culture medium for oocystis and preparation method of oocystis purebred liquid

Abstract

The invention relates to the technical field of aquaculture water quality manipulation and water environment governance, and particularly relates to a special culture medium for oocystis and a preparation method of oocystis purebred liquid. The preparation method comprises: inoculating purebred oocystis separated from a high-yield shrimp culture pond into the special culture medium; continuously carrying out aerated culture purification under the conditions of a temperature of 23 to 28 DEG C and illumination intensity of 1,800 to 2,000Lx; when concentration of the oocystis reaches 650,000 to 750,000 pieces per milliliter after 12 to 18 days, stopping aeration; after sedimentation, removing supernatant liquid to obtain the oocystis purebred liquid. Compared to the prior art, the invention provides a special nutrition formulation and the most proper physical and chemical factor culture conditions of a temperature, salinity, acidity and alkalinity, illumination and the like for oocystis culture, and the prepared oocystis purebred liquid can be used for selectively breeding and culturing oocystis with high activity and high stress resistance.

Description

technical field [0001] The invention relates to the technical field of aquaculture water quality regulation and water body environment treatment, and in particular to a preparation method for a special culture medium for oocysts and a pure oocyst liquid. Background technique [0002] Oocystis belongs to Chlorophyta, Trebouxiophyceae, Chlorellales, Oocystaceae. The plant body is a spherical or elliptical spherical group, and the group is usually composed of 2, 4 or 8 cells, and there is a gelatinous coating composed of pectin outside the group. The algae cells are oval or oval, with a length of 10-19 μm and a width of 9-13 μm, and generally produce 2, 4, 8 or 16 spore-like spores. Oocysts are mainly distributed in water bodies rich in organic matter such as estuaries and aquaculture ponds. They have the characteristics of long duration and stable population. They can reduce the concentration of harmful factors such as ammonia nitrogen and nitrite in water bodies, and increas...

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Problems solved by technology

[0004] The traditional way of cultivating oocysts is to cultivate in open cement ponds, which has a large scale of cultivation and high yield, but the disadvantages are: (1) It is easy to pollute, occupies a large area, is greatly affected by the weather, is not easy to control, and the growth of algae cells is slow , the doubling time is long, the one-time investment is large and the output is low, and it is not suitable for the expansion stage; (2) the algal phase of the oocysts cultivated by the traditional method is not stable enough, and it is easy to age and lose algae. It is especially suitable for shrimp and other culture ponds In the middle and late stages of cultivation, the water quality of the pond will be unstable, and the algae phase will be unfavorable for the growth of prawns, and the algae phenomenon will occur, and the dead algae will produce a large amount of algae toxins, which will lead to the death of seafood such as prawns; (3) The cultivated oocysts are difficult to store and transport for a long time at room temperature, causing inconvenience to use

These make it difficult for oocysts to be applied on a large scale in actual production

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