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Application of RBBP4 (Retinoblastoma Binding Protein 4) gene to clinical medication

A technology of gene and gene chip, which is applied in the direction of drug combination, pharmaceutical formula, microbial determination/inspection, etc., can solve problems such as lack of article description, achieve clear results, simplify the quantitative detection process, and quickly respond

Inactive Publication Date: 2018-05-22
QINGDAO MEDINTELL BIOMEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The rapid development and application of high-throughput sequencing technology has provided a more comprehensive and rapid analysis method for the study of the pathogenesis of osteoporosis. Liuwei Dihuang Pills have obvious curative effects on postmenopausal osteoporosis, but there are no articles to explain its effect. For the mechanism of action, the inventors selected candidate genes through high-throughput sequencing technology, which provided a theoretical basis for Liuwei Dihuang Pills to treat postmenopausal osteoporosis, and provided a basis for precise medication for postmenopausal osteoporosis

Method used

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  • Application of RBBP4 (Retinoblastoma Binding Protein 4) gene to clinical medication
  • Application of RBBP4 (Retinoblastoma Binding Protein 4) gene to clinical medication
  • Application of RBBP4 (Retinoblastoma Binding Protein 4) gene to clinical medication

Examples

Experimental program
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Effect test

Embodiment 1

[0049] Example 1 High-throughput sequencing and analysis

[0050] The peripheral blood samples of 3 postmenopausal osteoporosis patients before and after taking Liuwei Dihuang Pills and 3 healthy controls were collected for RNA extraction. After RNA extraction, agarose gel electrophoresis was performed. Whether the quality of the RNA sample is qualified or not, whether it can be used for further transcriptome analysis. Then, the extraction of RNA samples was detected by NanoDrop1000 spectrophotometer, and the sample requirements for RNA-seq sequencing: OD260 / OD280 was 1.8-2.2.

[0051] The sequencing platform is the HiSeq 2500 high-throughput sequencing platform of Illumina, which performs high-throughput transcriptome deep sequencing. After sequencing, we use Fast-QC software to evaluate the quality of the sequencing data as a whole, including the quality value distribution of bases, quality value position distribution, GC content, PCR duplication content, kmer frequency, et...

Embodiment 2

[0052] Example 2 Peripheral blood RBBP4 gene expression in postmenopausal osteoporosis patients before and after taking Liuwei Dihuang Pills

[0053] 1. Materials and methods

[0054] 1. Materials

[0055] The consent of 22 patients with postmenopausal osteoporosis was collected, and the peripheral blood was collected at the initial stage of testing. After taking Liuwei Dihuang Pills for half a year, the peripheral blood was collected again, and the samples were grouped and numbered.

[0056] 2. Method

[0057] 2.1 Extraction of total RNA from peripheral blood of postmenopausal osteoporosis and healthy people

[0058] use Reagent was used to extract RNA from samples, and the experimental operation was performed according to the product instructions.

[0059]Judgment criteria for RNA quality: the OD260 / OD280 value of the RNA sample is between 1.8 and 2.2; the electrophoretic pattern of total RNA has clear 28S and 18S bands; difference.

[0060] 2.2 Synthesis of cDNA by r...

Embodiment 3

[0084] Example 3 RBBP4 gene expression in peripheral blood of postmenopausal osteoporosis patients and healthy people

[0085] 1. Materials

[0086] The peripheral blood of 32 postmenopausal osteoporosis patients and 29 healthy people were collected, grouped and numbered.

[0087] 2. Method

[0088] Concrete steps refer to embodiment 3.

[0089] 3. Results

[0090] Real-time quantitative PCR result display (see figure 2 ): The results of qRT-PCR amplification are stable, and the expression level of RBBP4 in the peripheral blood of postmenopausal osteoporosis is higher than that of healthy people, the former is about 1.23 times that of the latter, the above results verify that Qualcomm Quantitative sequencing results.

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Abstract

The invention relates to application of an RBBP4 (Retinoblastoma Binding Protein 4) gene to clinical medication, and particularly relates to application of the RBBP4 gene to the detection of postmenopausal osteoporosis and the clinical medication of Liuwei Dihuang pill. The Liuwei Dihuang pill has an obvious curative effect on the postmenopausal osteoporosis; however, a mechanism of action of theLiuwei Dihuang pill is not currently expounded yet by an article, and the inventors, through high-throughput sequencing, pick out a coordinate gene, provide a theoretical foundation for treating the postmenopausal osteoporosis by the Liuwei Dihuang pill, and further provide a basis for the accurate medication of the postmenopausal osteoporosis.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of RBBP4 gene in clinical medicine, and more specifically to the application of RBBP4 gene in detection of postmenopausal osteoporosis and clinical medicine of Liuwei Dihuang Wan. Background technique [0002] Retinoblastoma binding protein 4 (RBBP4), a member of the WD repeat family, has 4 WD repeats in its molecular structure, which can bind to a putative functional domain of the retinoblastoma protein RB. named. RBBP4 is a member of several histone deacetylase complexes in vivo, involved in chromatin remodeling, such as NURD complex, SIN3 complex, CAF-1 complex, etc. RBBP4 is also a subunit of the PRC2 complex capable of methylating histone H3. RBBP4 is located at the core of these complexes and links other subunits of these complexes to histones to exert transcriptional repression through histone deacetylases. RBBP4 thus affects all stages of chromatin metabolism...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6883G01N33/68A61K45/00A61K31/7088A61P19/10
CPCA61K31/7088A61K45/00C12Q1/6883C12Q2600/106C12Q2600/158G01N33/6893G01N2333/47G01N2800/108G01N2800/52
Inventor 孙耀兰常鹏
Owner QINGDAO MEDINTELL BIOMEDICAL CO LTD
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