In-vitro tissue culture and propagation method of radix pueraiae plants

A technology of in vitro culture and pueraria, applied in the field of in vitro culture and reproduction of plant tissue, can solve problems such as single mode, and achieve the effect of rich nutrition

Active Publication Date: 2018-05-25
江苏雨霏农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different explants and explants are drawn at different times, and the suitable hormone combinations are also different. There are great differences, and the specific suitable hormone concentrations are also quite different.
Moreover, the commonly used hormones in the prior art are combined use of 6-BA and NAA, and the mode is relatively single

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Select potted excellent and robust kudzu vines and cultivate them in a greenhouse. When the vines grow to more than 1m, take materials. Take normal and mature kudzu vines, cut off the leaves and petioles, and cut them into 3-5cm long stems. The collected stems Rinse it with tap water and dry it, disinfect the surface with ethanol with a volume fraction of 70% in the ultra-clean workbench for 30 seconds, rinse with sterile water 2-3 times, then soak with 0.1% mercuric chloride for 8 minutes, and rinse with sterile water Rinse 4-5 times, dry the surface moisture with sterile filter paper, and obtain sterile stem sections; then cut the sterile stem sections into thin slices about 1 mm thick with a sterilized scalpel.

[0022] Thin slices were cut and then inoculated on the callus induction medium with the formula: MS+2,4-D 0.9mg / L+KT 0.3mg / L+sucrose 30g / L+6g / L agar, pH 5.9 , Culture temperature 20-22 ℃, culture under dark conditions.

[0023] After 4-5 days of culture, th...

Embodiment 2

[0027] Select potted excellent and robust kudzu vines and cultivate them in a greenhouse. When the vines grow to more than 1m, take materials. Take the vigorously growing semi-lignified kudzu vines, cut off the leaves and petioles, and cut them into 2-3cm long stems. Rinse the stem section with tap water and dry it, use 70% ethanol surface disinfection treatment with volume fraction in ultra-clean workbench for 20 seconds, rinse 1-2 times with sterile water, then soak for 5 minutes with 0.1% mercuric chloride, Rinse with sterile water for 3-4 times, blot the surface moisture with sterile filter paper, and obtain sterile stem segments; then cut the sterile stem segments into thin slices about 2 mm thick with a sterilized scalpel.

[0028] Cut the thin slices and inoculate them on callus induction medium with formula: MS+2,4-D 0.6mg / L+KT 0.1mg / L+sucrose 30g / L+6g / L agar, pH value 5.9 , Culture temperature 20-22 ℃, culture under dark conditions.

[0029] After 3-5 days of culture...

Embodiment 3

[0034] Select the vigorously growing Pueraria mirifica in the field, cut off the semi-lignified Pueraria vines from the vines of the year, cut off the leaves and petioles, cut into 2-3cm long stems, rinse them with tap water and thoroughly soak them in a solution added with detergent , then rinse it with tap water and dry it. In the ultra-clean workbench, use 70% ethanol to disinfect the surface for 30 seconds, rinse it with sterile water for 1-2 times, and then soak it in 0.1% mercury chloride for 6 minutes. , rinsed with sterile water for 3-4 times, and blotted the surface moisture with sterile filter paper to obtain sterile stem segments; then cut the sterile stem segments into thin slices about 3 mm thick with a sterilized scalpel.

[0035] Thin slices were cut and then inoculated on the callus induction medium with the formula: MS+2,4-D 0.8mg / L+KT 0.2mg / L+sucrose 30g / L+6g / L agar, pH value 5.9 , Culture temperature 20-22 ℃, culture under dark conditions.

[0036] After 3-...

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PUM

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Abstract

The invention relates to an in-vitro tissue culture and propagation method of radix pueraiae plants. By utilizing thin slices of stem segments of the radix pueraiae plants as explants, the method comprises the steps of induction of calluses, induction of adventitious buds, multiplication of the adventitious buds, rooting culture and culture of optional strong seedlings; through the utilization ofthe thin slices as the explants and under low plant hormones concentration, the calluses can be easily induced, a crude extract of fresh radix pueraiae is added in an adventitious bud induction culture medium, the better induced differentiation of adventitious buds from the calluses can be promoted, and the better growth and development of the adventitious buds is facilitated.

Description

technical field [0001] The invention belongs to the field of in vitro culture and propagation of plant tissues, and more specifically relates to a method for in vitro culture and propagation of kudzu root plants. Background technique [0002] Pueraria lobata usually refers to the root tuber of the plant Pueraria lobata, and the root tuber of the plant Puerariae lobata var. Thomsonii can also be used. Both plants are Pueraria plants of the Leguminosae family and are perennial vines. Pueraria lobata is a high-quality plant material for both medicine and food. Its roots, stems, leaves, and flowers can all be used as medicine. As a traditional Chinese medicine, kudzu root is used for the treatment of various diseases, such as: acute myocardial infarction, unstable angina pectoris, hyperviscosity, acute cerebral infarction, cervical spondylosis and other diseases. Therefore, there is a great demand for kudzu root. The traditional propagation methods of Pueraria lobata are seed ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 孙勇周长生孙永妹
Owner 江苏雨霏农业科技发展有限公司
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