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New mechanism of anti-alcoholic hepatic injury by wolfberry red element and application of hepatoprotective products

A technology of alcoholic liver injury and lycium ruberin, which is applied in the fields of medicine, special medical food, and health food, can solve the problems of insignificant detoxification and liver protection, long course of disease, and many side effects.

Active Publication Date: 2018-05-25
百瑞源枸杞股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many drugs for the treatment of liver injury on the market, but most western medicines have many side effects, long course of disease, etc., and have no obvious effect on detoxification and liver protection. Long-term use of these drugs is prone to fullness, abdominal pain, diarrhea, and appetite Adverse reactions such as drop, nausea, vomiting, allergic reaction, intestinal flora disorder
At present, many scholars have studied the treatment of alcoholic liver disease with traditional Chinese medicine, such as silymarin capsules, pazhu pills, acai berries, scallion polysaccharides and barley greens, etc. to treat liver damage and improve liver function, but many of them have not yet been developed into clinical drugs

Method used

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  • New mechanism of anti-alcoholic hepatic injury by wolfberry red element and application of hepatoprotective products
  • New mechanism of anti-alcoholic hepatic injury by wolfberry red element and application of hepatoprotective products
  • New mechanism of anti-alcoholic hepatic injury by wolfberry red element and application of hepatoprotective products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Example 1 The protective effect of Lycium barbarum on rat AFLD model

[0069] 60 rats were randomly selected and divided into 4 groups. They were blank control group, AFLD model group, positive control group and treatment group.

[0070] For the AFLD model group and the treatment group, the model of alcoholic liver injury in rats was established by intragastric administration of alcohol. The concentration of 1.25% alcohol was stimulated on the first day, the concentration was increased to 1.67% on the second day, and the concentration was increased on the third and fourth days. to 2.5%, then give 5% alcohol by gavage until 4 weeks. The blank control group and the positive control group used dextrin or maltose instead of alcohol to ensure that the two groups of rats consumed the same amount of calories.

[0071] After 14 days of alcohol gavage, the positive control group and the treatment group were given 5mg / kg wolfberry red element (dissolved in PBS containing 5% Twe...

Embodiment 2

[0079] Example 2 The protective effect of Lycium barbarum on autophagy inhibition or receptor knockdown rats

[0080] 1. Models are grouped as shown in Table 2, with 15 animals in each group.

[0081] Table 2: Grouping processing methods

[0082]

[0083] Knockdown of receptors:

[0084] For mice with liver-specific knockdown of P2X7 receptor, Adiponectin receptor 1 and insulin receptor, 1 × 10 12 Genomic copies of AAV8 were coated with respective shRNAs to knockdown the expression of target genes in the liver. Sixty knockdown rats were constructed for each receptor. For the group of rats whose systemic "autophagy" was inhibited, Chloroquine (CQ, chloroquine) (60mg / kg) was intraperitoneally injected on the first day of AFLD modeling. 60 autophagy inhibited rats were constructed.

[0085] Construction of the AFLD model:

[0086] They were blank control group, AFLD model group, positive control group and treatment group. For the AFLD model group and the treatment group...

Embodiment 3

[0097] Rat normal liver cell line BRL-3A (purchased from ATCC, USA) was used and divided into 6 groups. Respectively, blank control group, alcohol treatment group, alcohol + wolfberry red pigment group, inhibitor control group, inhibitor + alcohol group, alcohol + inhibitor + wolfberry red pigment group.

[0098] Among them, the inhibitor group was divided into four groups, which were given respectively:

[0099] Autophagy inhibitor 3-MA (purchased from Sigma, USA),

[0100] P2X7 receptor-specific siRNA (purchased from Sigma, USA),

[0101] Adiponectin receptor 1-specific siRNA (purchased from Sigma, USA);

[0102] Insulin receptor-specific siRNA (purchased from Sigma, USA).

[0103] When the BRL-3A cells were cultured to a density of 60%, inhibitors were firstly added to the corresponding groups at concentrations of 3-MA (10 mM) and three siRNAs (both 100 nM). After 12 hours, add 250 nM alcohol and / or 1 μM Lycirubin and treat for 24 hours. Then, the cell viability was dete...

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Abstract

The invention relates to the field of medicines, health foods and special dietary products, and particularly relates to a new mechanism of anti-alcoholic liver injury by a wolfberry red element. According to the new mechanism of the anti-alcoholic liver injury by the wolfberry red element, experiments show that a self-phagocytic response promoter, a P2X7 receptor agonist, an Adiponectin receptor 1agonist, and an insulin receptor agonist can all play an important role in improving alcoholic liver injury. The self-phagocytic promoter, the P2X7 receptor agonist, the Adiponectin receptor 1 agonist, and the insulin receptor agonist screened by the new mechanism are the wolfberry red element. The invention also provides a composition comprising the wolfberry red element, and the composition hasa good effect of improving the alcoholic liver injury.

Description

technical field [0001] The invention relates to the fields of medicine, food for special medical treatment and health food, in particular to the new mechanism of medlar red pigment in resisting alcoholic liver injury and its application in liver protection products. Background technique [0002] Alcoholic liver injury or Alcoholic liver disease (Alcoholic liver disease, ALD) is a liver damage lesion caused by long-term heavy drinking. leading cause of liver damage. The pathogenic factor of ALD is single and clear, that is, it is caused by long-term intake of large amounts of alcohol, but the pathogenesis of ALD is very complicated and has not yet been elucidated. Some studies suggest that the pathogenesis of ALD is related to the toxic effect of alcohol and its metabolites, immune mediation, various cytokines, endotoxin and apoptosis. At present, some scholars put forward the "second hit" theory, which believes that the fatty degeneration of liver cells caused by alcohol a...

Claims

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Application Information

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IPC IPC(8): A61K31/232A61K31/047A61K31/015A61P1/16A23L33/12
CPCA23V2002/00A61K31/015A61K31/047A61K31/232A61K2300/00A23V2200/30A23V2250/18
Inventor 肖佳苏国辉高昊郭雨桐张金宏郝向峰李静静
Owner 百瑞源枸杞股份有限公司
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