Biodegradable and environment friendly glass cleaning solution
A glass cleaning and biodegradation technology, applied in the direction of anti-corrosion composition, detergent compounding agent, detergent composition, etc., can solve problems such as soil and basement pollution
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0026] A ring of Bacillus subtilis LPB-3 strain was inoculated into LB test tube slant medium for activation, the culture temperature was 32°C, and the culture was statically cultured for 24 hours. The activated slant strain was transferred into a 2L shake flask for primary seed expansion cultivation at a cultivation temperature of 32° C., a shaking table rotation speed of 150 rpm, and a cultivation period of 10 h. Put the primary seed liquid into a 10L seed tank according to the inoculum amount of 1% for secondary seed expansion cultivation, the cultivation temperature is 32°C, the rotation speed is 100rpm, the ventilation volume is 1vvm, the tank pressure is 0.02MPa, and the cultivation period is 8h. According to the inoculum amount of 5%, the secondary seed solution was put into a 100L fermenter for fermentation and cultivation. The cultivation temperature was 32°C, the initial pH was 6.5, the rotation speed was 50rpm, the ventilation rate was 0.5vvm, the tank pressure was 0...
Embodiment 2
[0028] Take a ring of Bacillus subtilis LPB-3 strain and inoculate it in the slant medium of LB test tube for activation. The culture temperature is 35°C, and the culture is static for 24 hours. The activated slant strain was transferred into a 2L shake flask for primary seed expansion cultivation at a cultivation temperature of 35° C., a shaking table rotation speed of 200 rpm, and a cultivation period of 15 hours. According to the inoculum amount of 4%, the primary seed solution was put into a 10L seed tank for secondary seed expansion cultivation. The cultivation temperature was 35°C, the rotational speed was 150rpm, the ventilation rate was 1.5vvm, the tank pressure was 0.04MPa, and the cultivation period was 12h. According to the inoculum amount of 8%, the secondary seed solution was inserted into a 100L fermenter for fermentation and cultivation. The cultivation temperature was 35°C, the initial pH was 7.0, the rotation speed was 100rpm, the ventilation rate was 1vvm, the...
Embodiment 3
[0030]Take a ring of Bacillus subtilis LPB-3 strain and inoculate it in the slant medium of LB test tube for activation. The culture temperature is 35°C, and the culture is static for 24 hours. The activated slant strain was transferred into a 2L shake flask for primary seed expansion cultivation at a cultivation temperature of 35° C., a shaking table rotation speed of 150 rpm, and a cultivation period of 12 hours. Put the primary seed liquid into a 10L seed tank according to the inoculum amount of 5% for secondary seed expansion cultivation, the cultivation temperature is 35°C, the rotation speed is 100rpm, the ventilation volume is 1vvm, the tank pressure is 0.04MPa, and the cultivation period is 8h. According to the inoculation amount of 10%, the secondary seed solution was inserted into a 100L fermenter for fermentation and cultivation. The cultivation temperature was 35°C, the initial pH was 6.5, the rotation speed was 100rpm, the ventilation rate was 1vvm, the tank pressu...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com