Biodegradable and environmentally friendly glass cleaning fluid
A glass cleaning and biodegradation technology, applied in the direction of anti-corrosion composition, detergent composition, detergent compounding agent, etc., can solve the problems of soil and basement pollution, etc.
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Embodiment 1
[0026] A ring of Bacillus subtilis LPB-3 strain was inoculated into LB test tube slant medium for activation, the culture temperature was 32°C, and the culture was statically cultured for 24 hours. The activated slant strain was transferred into a 2L shake flask for primary seed expansion cultivation at a cultivation temperature of 32° C., a shaking table rotation speed of 150 rpm, and a cultivation period of 10 h. According to the inoculum amount of 1%, the primary seed solution was put into a 10L seed tank for secondary seed expansion cultivation. The cultivation temperature was 32°C, the rotation speed was 100rpm, the ventilation rate was 1vvm, the tank pressure was 0.02MPa, and the cultivation period was 8h. According to the inoculum amount of 5%, the secondary seed solution was put into a 100L fermenter for fermentation and cultivation. The cultivation temperature was 32°C, the initial pH was 6.5, the rotation speed was 50rpm, the ventilation rate was 0.5vvm, the tank pres...
Embodiment 2
[0028] Take a ring of Bacillus subtilis LPB-3 strain and inoculate it in the slant medium of LB test tube for activation. The culture temperature is 35°C, and the culture is static for 24 hours. The activated slant strain was transferred into a 2L shake flask for primary seed expansion cultivation at a cultivation temperature of 35° C., a shaking table rotation speed of 200 rpm, and a cultivation period of 15 hours. Put the primary seed liquid into a 10L seed tank according to the inoculation amount of 4% for secondary seed expansion cultivation, the cultivation temperature is 35°C, the rotation speed is 150rpm, the ventilation rate is 1.5vvm, the tank pressure is 0.04MPa, and the cultivation period is 12h. According to the inoculum amount of 8%, the secondary seed solution was put into a 100L fermenter for fermentation and cultivation. The cultivation temperature was 35°C, the initial pH was 7.0, the rotation speed was 100rpm, the ventilation volume was 1vvm, the tank pressure...
Embodiment 3
[0030]Take a ring of Bacillus subtilis LPB-3 strain and inoculate it in the slant medium of LB test tube for activation. The culture temperature is 35°C, and the culture is static for 24 hours. The activated slant strain was transferred into a 2L shake flask for primary seed expansion cultivation at a cultivation temperature of 35° C., a shaking table rotation speed of 150 rpm, and a cultivation period of 12 hours. Put the primary seed liquid into a 10L seed tank according to the inoculum amount of 5% for secondary seed expansion cultivation, the cultivation temperature is 35°C, the rotation speed is 100rpm, the ventilation volume is 1vvm, the tank pressure is 0.04MPa, and the cultivation period is 8h. According to the inoculation amount of 10%, the secondary seed solution was inserted into a 100L fermenter for fermentation and cultivation. The cultivation temperature was 35°C, the initial pH was 6.5, the rotation speed was 100rpm, the ventilation rate was 1vvm, the tank pressu...
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