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Method for recovering ampicillin from enzymatic synthesis of ampicillin mother liquor

A technology of ampicillin mother liquor and ampicillin mother, applied in the direction of organic chemistry, etc., can solve the problems of power and other material loss, large ampicillin loss, low treatment waste liquid, etc., so as to reduce related equipment and materials and ensure biological activity. and high yield and high efficiency

Active Publication Date: 2019-04-26
SHANXI WEIQIDA PHARMA IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The processing method of ampicillin mother liquor mainly contains two kinds at present: one, use macroporous resin adsorption column to reclaim and purify ampicillin, although this method has avoided the use of toxic substances and the hydrolysis recovery of ampicillin, its treatment waste liquid amount is relatively large Low, in addition, the method cost of using macroporous adsorption resin is higher, and flow process is also relatively loaded down with trivial details; Two, use hydrolytic enzyme that ampicillin is hydrolyzed to generate 6-APA and D-phenylglycine, then reclaim respectively, but this method is for The production of ampicillin has a large loss, which is also the loss of power and other materials in the early stage of production

Method used

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  • Method for recovering ampicillin from enzymatic synthesis of ampicillin mother liquor
  • Method for recovering ampicillin from enzymatic synthesis of ampicillin mother liquor
  • Method for recovering ampicillin from enzymatic synthesis of ampicillin mother liquor

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Embodiment 1

[0053] This embodiment is used to illustrate the method for reclaiming ampicillin from the mother liquor of enzymatically synthesized ampicillin provided by the present invention

[0054] 1) Measure 30L of the ampicillin crystallization mother liquor, the content of ampicillin in it is 7.3mg / ml by liquid phase detection, and the temperature of the mother liquor is controlled at 10.5-11.5°C.

[0055] 2) Turn on the stirring, set the rotation speed at 300rpm, start to drop 3M hydrochloric acid and slowly adjust the pH of the feed solution to 1.5.

[0056] 3) Use a pump to inject the material into the 230 molecular weight nanofiltration membrane, and adjust the concentration pressure to 3MPa.

[0057] 4) Turn on the temperature control equipment to control the temperature of the film at 14.5-15.5°C.

[0058] 5) Return the concentrated solution to the storage tank, collect the filtrate into the filtrate collection tank, and concentrate to the concentration of 8 times of the ampic...

Embodiment 2

[0064] This embodiment is used to illustrate the method for reclaiming ampicillin from the mother liquor of enzymatically synthesized ampicillin provided by the present invention

[0065] 1) Get the same ampicillin crystallization mother liquor as in Example 1, and control the temperature of the mother liquor to be 15.5-16° C.

[0066] 2) Turn on the stirring, set the rotation speed at 250 rpm, start to drop 3M hydrochloric acid and slowly adjust the pH of the feed solution to 1.3.

[0067] 3) Use a pump to inject the material into the 160 molecular weight nanofiltration membrane, and adjust the concentration pressure to 2.5MPa.

[0068] 4) Turn on the temperature control equipment, and control the temperature of the film at 10.5-11.5°C.

[0069] 5) Return the concentrated solution to the storage tank, collect the filtrate into the filtrate collection tank, and concentrate to a concentration 9 times that of the ampicillin crystallization mother liquor.

[0070] 6) Transfer t...

Embodiment 3

[0075] This embodiment is used to illustrate the method for reclaiming ampicillin from the mother liquor of enzymatically synthesized ampicillin provided by the present invention

[0076] 1) Get the same ampicillin crystallization mother liquor as in Example 1, and control the temperature of the mother liquor to be 12.5-13.5°C.

[0077] 2) Turn on the stirring, set the rotation speed to 300rpm, and start to drop 3M hydrochloric acid to slowly adjust the pH of the feed solution to 1.8.

[0078] 3) Use a pump to inject the material into the 130 molecular weight nanofiltration membrane, and adjust the concentration pressure to 2MPa.

[0079] 4) Turn on the temperature control equipment to control the temperature of the film at 8.5-9.5°C.

[0080] 5) Return the concentrated solution to the storage tank, collect the filtrate into the filtrate collection tank, and concentrate to a concentration 7 times that of the ampicillin crystallization mother liquor.

[0081] 6) Transfer the ...

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Abstract

The invention relates to the field of recycling of ampicillin, in particular to a method for recycling ampicillin from an enzyme synthesis type ampicillin mother liquor. The method comprises the following steps of (1) separating the acidified ampicillin mother liquor by a film, so as to obtain a concentrated solution of the ampicillin; (2) enabling the obtained concentrated solution of the ampicillin in step (1) to be in contact with an alkaline regulator, and crystallizing, so as to obtain a crystal slurry of the ampicillin. The ampicillin obtained by the method has the characteristics that the purity is high, and the recycling rate is high; the preparation technology is simple, the cost is lower, and more environment-friendly effect is realized; under more preferable conditions, such asthe stirring condition, the crystal is separated out, and the stirring phase is set to the phase 2 or phase 3, so that the granularity of the obtained ampicillin crystal is more uniform.

Description

technical field [0001] The invention relates to the field of ampicillin recovery, in particular to a method for recovering ampicillin from mother liquor of ampicillin synthesized by enzymatic method. Background technique [0002] Ampicillin is a beta-lactam antibiotic that treats a variety of bacterial infections. Indications include respiratory infections, urinary tract infections, meningitis, salmonellosis, and endocarditis. [0003] The processing method of ampicillin mother liquor mainly contains two kinds at present: one, use macroporous resin adsorption column to reclaim and purify ampicillin, although this method has avoided the use of toxic substances and the hydrolysis recovery of ampicillin, its treatment waste liquid amount is relatively large Low, in addition, the method cost of using macroporous adsorption resin is higher, and flow process is also relatively loaded down with trivial details; Two, use hydrolytic enzyme that ampicillin is hydrolyzed to generate 6...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D499/18C07D499/68
CPCC07D499/18C07D499/68
Inventor 王启斌田伟陈琪侯瑞峰路锐张华秀
Owner SHANXI WEIQIDA PHARMA IND
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