Terahertz markless detection method and apparatus for biochemical liquid sample

Abstract

The invention discloses a terahertz markless detection method for a biochemical liquid sample and provides a detection device and a manufacturing process thereof. The detection device comprises a terahertz rectangular waveguide resonant cavity, a micro-fluidic channel and a detector slot. A micro-fluidic tube is inserted into the micro-fluidic channel; and a terahertz detector is inserted in the detector slot. A terahertz wave is emitted at one side of the waveguide resonant cavity; a to-be-detected sample is introduced into the micro-fluidic channel and disturbance of an electromagnetic fieldin the waveguide resonant cavity is caused, so that the resonant frequency and quality factor of the resonant cavity change; and the detector detects changes of the resonant frequencies and quality factors of the waveguide resonant cavity before and after adding of the sample to obtain a complex permittivity of the to-be-detected sample. According to the invention, with the terahertz technology,the biochemical liquid sample is detected. The terahertz markless detection method has characteristics of high detection sensitivity, high detection precision, and small sample demand amount; and thedetection device has advantages of high integration, miniaturization, and high portability and has the broad application prospects.

Description

technical field [0001] The invention relates to detection technology of biochemical samples, in particular to a terahertz label-free detection method and device. Background technique [0002] So far, most of the detection methods widely used in biochemical samples are labeled detection methods based on fluorescent or isotope markers. Through the detection of external markers, the properties and characteristics of the original target molecules can be inferred indirectly. Although this labeled detection method has the advantages of simplicity, convenience, easy operation, fast imaging speed, and high spatial resolution, the introduction of labeled molecules inevitably destroys the structure and activity of target source molecules, and the efficiency of fluorescence emission varies with the reaction. Different substances are different, and there are also problems such as photobleaching. Therefore, the above bottlenecks limit the further development of labeled detection methods...

AI Technical Summary

Problems solved by technology

At present, the label-free detection of biochemical samples using terahertz technology is mostly realized through terahertz time-domain spectroscopy systems, but this method is mainly aimed at solid samples, usually requires a large number of samples, and the spectral resolution and spatial resolution are relatively low. Low, low detection accuracy and precision, in addition, the experimental device is bulky, poor in integration and portability, and not very practical in many actual on-site diagnostic applications

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