Perfluorocarbon albumin nanoparticles and preparation method and application thereof

An albumin nanoparticle and perfluorocarbon technology, which is applied in the field of preparation of albumin nanoparticles, can solve the problems of high requirements, influence drug safety, and limit the application of preparations related to albumin nanoparticles, and achieves a simple and easy preparation process. , the effect of improving biological safety and low equipment requirements

Active Publication Date: 2018-06-05
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cross-linking method needs to introduce a highly toxic cross-linking agent, which seriously affects the safety of medication
The high-pressure homogenization method has extremely high requirements on equipment and the prepared albumin nanoparticles have weak loading capacity
These deficiencies limit the clinical application of albumin nanoparticle-related preparations

Method used

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  • Perfluorocarbon albumin nanoparticles and preparation method and application thereof
  • Perfluorocarbon albumin nanoparticles and preparation method and application thereof
  • Perfluorocarbon albumin nanoparticles and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Preparation of perfluorotributylamine (PFTBA)-albumin nanoparticles:

[0042] Measure 0.5mL of human serum albumin stock solution (10g / 50mL) into a 7mL vial, add deionized water to dilute to 2mL, and mix well at 20°C. Then add 1.5mL perfluorotributylamine (PFTBA), then add 1.0mL ethanol (analytical grade), and mix well. Use an ultrasonic probe to go deep into the vial to the bottom of the liquid, use 500W power during ultrasonication, set the ultrasound to stop for 3s after every 2s, ultrasonication 3 times, each time 2 minutes, and the preparation needs to be placed in a 20°C water bath after each ultrasonication 1-2 minutes. Transfer the sample to a 4mL centrifuge tube, centrifuge at 3000rpm for 3min, and take the supernatant, which is the prepared perfluorotributylamine albumin nanoparticles.

[0043]The morphology of perfluorotributylamine-albumin nanoparticles was observed by transmission electron microscope (TEM). First, the prepared preparation was diluted 160...

Embodiment 2

[0045] Preparation of perfluorooctyl bromide (PFOB)-albumin nanoparticles:

[0046] Measure human serum albumin stock solution and add water to dilute to albumin concentration of 5g / 100mL, and mix well at 25°C. Take 2 mL of the above-mentioned diluted solution, add 3 g of perfluorooctyl bromide (PFOB), and then add 1.0 mL of ethanol (analytical grade), and mix well. Use an ultrasonic probe to penetrate deep into the bottle to near the bottom of the liquid for sonication. Transfer the sample to a centrifuge tube, centrifuge at 3000rpm for 3min, and take the supernatant, which is the prepared perfluorooctyl bromide albumin nanoparticles with an average particle size of 220nm.

Embodiment 3

[0048] Preparation of perfluorotripropylamine (PFTPA) + perfluorodecalin (FDC)-albumin nanoparticles:

[0049] Measure human serum albumin stock solution and add water to dilute to albumin concentration of 5g / 100mL, and mix well at 30°C. Take 2 mL of the above diluted solution, add 0.33 g of perfluorotripropylamine and 0.79 g of perfluorodecalin, then add 1.0 mL of ethanol (analytical grade), and mix well. Use an ultrasonic probe to penetrate deep into the bottle to near the bottom of the liquid for sonication. Transfer the sample to a centrifuge tube, centrifuge at 3000rpm for 3min, and take the supernatant, which is the prepared perfluorotripropylamine perfluorodecalin albumin nanoparticles, with an average particle size of 150nm.

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Abstract

The invention discloses perfluorocarbon albumin nanoparticles which comprise albumins and perfluorocarbon compounds, wherein the albumins coat the perfluorocarbon compounds, and the mass ratio of theperfluorocarbon compounds to the albumins is (4-40): 1. The perfluorocarbon albumin nanoparticles have good biological safety as a novel blood platelet inhibitor. The perfluorocarbon albumin nanoparticles which are administrated will induce great reduction of quantity of blood platelets in a human body, various functions of the blood platelets can be also inhibited obviously, and the perfluorocarbon albumin nanoparticles have relatively good clinical applications and practical treatment meaning.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a preparation method of albumin nanoparticles based on inhibition of platelet function. Background technique [0002] Platelet is a small piece of cytoplasm with biological activity, which is derived from the cleavage and shedding of mature megakaryocyte cytoplasm, and is of great significance to the realization of physiological and pathological hemostasis, thrombosis, inflammatory response, wound healing and other processes in the body. The process of mutual adhesion between platelets is platelet aggregation, which participates in physiological or pathological hemostasis, and it belongs to the process of blood coagulation. Thrombocytosis can cause abnormalities in the body. Thrombocytosis is often secondary to polycythemia, chronic myeloid leukemia, acute bleeding or hemolysis, malignant tumors, myelofibrosis, and splenectomy. [0003] Clinical investigations have shown that the occu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/38A61K9/50A61K47/06A61P7/02A61P19/08A61P35/00
CPCA61K9/5015A61K38/385
Inventor 胡一桥吴锦慧黄晴张宝丽
Owner NANJING UNIV
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