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Peony in vitro culture brown stain prevention rooting promoting agent

An in vitro culture and anti-browning agent technology, which is applied in plant regeneration, horticulture, botany equipment and methods, etc., can solve the problems of low reproduction coefficient, difficulty in achieving standardized large-scale production, and large individual differences

Inactive Publication Date: 2018-06-12
安徽心缘康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seedlings of peony have common variations and large individual differences, and it is difficult to maintain the excellent traits of the mother plant. The methods of asexual reproduction, grafting, and branching are limited by propagation materials, seasons, etc., and the reproduction coefficient is low. The few ornamental plants in the garden are propagated, and it is difficult to achieve standardized large-scale production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] An anti-browning and rooting-promoting agent for in vitro culture of peony, the components of which include by weight: 1 part of 6-benzylaminopurine, 0.05 part of indole-3-acetic acid, 0.5 part of diethyl hexanoate, and 35 parts of an anti-browning agent , 100 parts of activated carbon, 15 parts of indole butyric acid, 25 parts of carbon source.

Embodiment 2

[0018] An anti-browning and root-promoting agent for in vitro culture of peony, the components of which include by weight: 1 part of 6-benzylaminopurine, 0.1 part of indole-3-acetic acid, 1 part of diethyl diethylhexanoate, and 28 parts of anti-browning agent , 80 parts of activated carbon, 12 parts of indole butyric acid, 20 parts of carbon source;

[0019] Wherein, the anti-browning agent includes vitamin C and riboflavin; the mass ratio of vitamin C to riboflavin is 2:3; the carbon source is sucrose; the concentration of the sucrose is 20g / L; The cultivation temperature of the root-promoting agent is 24° C.; the pH value of the culture medium using the root-promoting agent is 6.0.

Embodiment 3

[0021] An anti-browning root-promoting agent for in vitro culture of peony, the components of which include by weight: 0.5 parts of 6-benzylaminopurine, 0.05 parts of indole-3-acetic acid, 2 parts of diethyl diethylhexanoate, and 35 parts of anti-browning agent , 50 parts of activated carbon, 10 parts of indole butyric acid, 30 parts of carbon source;

[0022] Wherein, the anti-browning agent includes vitamin C and riboflavin; the mass ratio of vitamin C and riboflavin is 3:4; the carbon source is sucrose; the concentration of the sucrose is 30g / L; The culture temperature of the root-promoting agent is 23° C.; the pH value of the culture medium using the root-promoting agent is 6.2.

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PUM

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Abstract

The invention discloses a peony in vitro culture brown stain prevention rooting promoting agent. The rown stain prevention and rooting promoting agent is prepared from the components of, in parts by weight:, 0.5-2 parts of 6-benzylaminopurine, 0.05-0.2 part of indol-3-acetic acid, 0.5-2 parts of diethyl aminoethyl hexanoate, 25-35 parts of brown stain prevention agents, 50-100 parts of active carbon, 10-20 parts of indolebutyric acid and 10-40 parts of carbon sources. Through appropriate proportion matching of the components, the peony in vitro culture brown stain prevention rooting promotingagent relieves the brown stain situation of adventitious buds, reduces the brown stain rate, promotes the proliferation and differentiation of the adventitious buds, induces rooting of the adventitious adventitious buds, and improves the success rate of tissue culture, and accordingly an explant can grow normally.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to an anti-browning and root-promoting agent for in vitro culture of peony. Background technique [0002] Peony is a small deciduous shrub of Ranunculaceae, which is a rare and unique flower in my country. Moutan root bark is used for medicinal purposes. It is a commonly used Chinese herbal medicine. It has the functions of clearing heat and dispersing blood stasis, removing fire, reducing swelling and pain, antibacterial, anticonvulsant and antioxidative. Peony seed endosperm is rich in fat, the unsaturated fatty acid content is as high as 92%, and its α-linolenic acid content is more than 40%, which has the functions of strengthening the brain and enhancing immunity. Peony has strong stress resistance, wide adaptability, single flower, large fruiting capacity, and seed reproduction. It is mainly used as ornamental peony grafting rootstock and traditional Chinese medicine p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 沈智配
Owner 安徽心缘康生物科技有限公司