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Complex microbial inoculant as well as preparation method and application thereof

A technology of compound bacterial agent and pediococcus, which is applied in the field of animal husbandry, can solve the problems of dry matter loss and low fermentation quality, and achieve the effects of strong survival ability, large lactic acid production, and high specificity

Active Publication Date: 2018-06-12
SHANXI AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of severe dry matter loss and low fermentation quality in corn ear silage, the present invention provides a composite bacterial agent, and further provides the application of the composite bacterial agent in corn ear silage

Method used

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  • Complex microbial inoculant as well as preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0021] Rhamnosus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 37 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain Lactobacillus rhamnosus suspension;

[0022] The ethanol-resistant Pediococcus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 33 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain ethanol-resistant Pediococcus suspension;

[0023] Lactobacillus plantarum was activated and cultured at 37 °C for 18 h, and the activated bacterial solution was inoculated into the MRS broth enrichment medium, cultured in the dark at 37 °C, and cultured on a constant temperature shaker at 150 rpm so that the final number of viable bacteria reached 5×10 10 CFU / ml, to obtain a suspension of Lactobacillus plantarum;

[0024] Lactobacillus Brucella was activated and cultured at 37 °C for 18 ...

Embodiment 2

[0034] Rhamnosus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 37 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain Lactobacillus rhamnosus suspension;

[0035] After the ethanol-resistant Pediococcus was activated and cultured at 34 °C for 18 h, it was inoculated into MRS medium, and cultured statically at 33 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain ethanol-resistant Pediococcus suspension;

[0036] The obtained Lactobacillus rhamnosus suspension and the ethanol-resistant Pediococcus suspension are mixed according to a volume ratio of 1:1.5 to prepare a composite bacterial agent;

[0037] Harvest the complete ear of corn with bracts in the milk ripening stage, and use a hammer feed mill to crush the fresh samples until the grains are broken, and add the above-mentioned compound bacterial agent at a dosage of 1×10 5 CFU / ...

Embodiment 3

[0039] Rhamnosus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 37 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain Lactobacillus rhamnosus suspension;

[0040] The ethanol-resistant Pediococcus was activated and cultured at 34 °C for 18 h, inoculated into MRS medium, and cultured statically at 33 °C for 24 hours, so that the final number of viable bacteria reached 3.5×10 9 CFU / ml, to obtain ethanol-resistant Pediococcus suspension;

[0041] The obtained Lactobacillus rhamnosus suspension and the ethanol-resistant Pediococcus suspension are mixed according to a volume ratio of 1:1 to prepare a composite bacterial agent;

[0042] Harvest the complete ear of corn with bracts in the milk ripening stage, and use a hammer feed mill to crush the fresh samples until the grains are broken, and add the above-mentioned compound bacterial agent at a dosage of 1×10 5 CFU / g, mixed evenly...

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Abstract

The invention discloses a complex microbial inoculant as well as a preparation method and application thereof, belongs to the technical field of animal husbandry, relates to silage, and solves the problems that corncob silage is severe in dry substance loss and is low in fermentation quality. The complex microbial inoculant is prepared from lactobacillus rhamnosus ZXY7 with a preservation number of CGMCC NO.11849 and a preservation date of December 9, 2015 and pediococcus ethanolidurans NO.1 with a preservation number of CGMCC NO.11850 and a preservation date of December 9, 2015. The complex microbial inoculant can remarkably reduce generation of alcohol or volatile substances, is high in dry matter storage rate, and can be widely applied to other silages with high sugar content.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry and relates to silage, in particular to a compound microbial agent, a preparation method thereof and an application in corn cob silage. Background technique [0002] Silage additives are an important part of the silage production process, which determines the quality of silage, and the quality of silage determines the quality of livestock products. [0003] About 50% of the silage in the domestic dairy farming industry is used in the form of TMR, and herbivores such as beef cattle and sheep are mainly fed separately. Whole plant corn silage is widely used in various dairy and breeding enterprises in my country. In order to achieve the purpose of effective feed utilization, secondary fermentation inhibitors are usually added to corn silage, acid agents and bacterial agents are added to alfalfa silage, etc. . Yeast can cause aerobic spoilage while relatively increasing the protein of silag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/04A23K30/18C12R1/225C12R1/01
CPCA23K30/18C12N1/04C12N1/20
Inventor 许庆方玉柱熊乙欧翔马菱艺高占军
Owner SHANXI AGRI UNIV
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