Functional molecular marker of related genes of cell nuclear male sterility of peppers and application thereof

A technology with the same function as the nucleus, which is applied in the determination/testing of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., and can solve the problems of fine positioning of pepper GMS fertility genes and no molecular markers, etc.

Active Publication Date: 2018-06-12
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no fine mapping of pepper GMS fertility genes at home and abroad, and there is no molecular marker that can be practically applied in breeding work. The selection and transfer of pepper GMS dual-purpose lines still adopts the classic backcross breeding method. If there are two generations a year, the cycle of breeding dual-purpose lines is as long as 6-7 years, and if GMS-related molecular markers can be developed, the time of breeding and transfer can be shortened to 3 years, which can significantly improve Improve the efficiency of breeding

Method used

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  • Functional molecular marker of related genes of cell nuclear male sterility of peppers and application thereof
  • Functional molecular marker of related genes of cell nuclear male sterility of peppers and application thereof
  • Functional molecular marker of related genes of cell nuclear male sterility of peppers and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Example 1, Development of Molecular Marker InDel-12 and Polymorphism Detection

[0081] 1. Development of Molecular Marker InDel-12

[0082] 1. Statistically locate the pollen fertility of each individual plant in the population

[0083] Using the capsicum male sterile line 09C460AB as the test material (fertile plants and sterile plants are near-isogenic lines), a mapping population consisting of 1110 individual plants was obtained. The pollen fertility of each individual plant in the positioning population was counted separately.

[0084] 2. Development of molecular marker InDel-12

[0085] (1) Take the fertile individual plants of the positioning population obtained in step 1, and mix the pools to obtain a fertile pool. The number of individual plants in each fertile pool is 30.

[0086] (2) Take the sterile individual plants of the positioning population obtained in step 1, and mix the pools to obtain sterile pools. The number of single plants in each sterile p...

Embodiment 2

[0119] Embodiment 2, application of molecular marker InDel-12

[0120] 1. Application 1

[0121] The peppers to be tested were 46 pepper inbred lines and varieties whose genotype was known as MsMs (see Table 1). All pepper inbred lines and varieties are publicly available from the College of Horticulture, China Agricultural University.

[0122] Table 1

[0123]

[0124]

[0125] 1. Carry out genotyping of the pepper to be tested according to the method of polymorphism detection one in Example 2.

[0126] 2. Plant the pepper samples to be tested in the field, manage them in the field routinely, and observe the pollen fertility after maturity.

[0127] For the experimental results of step 1, see figure 2 (M is a DNA marker, F is a fertile pool, S is a sterile pool, and the rest are 46 pepper inbred lines and varieties). The results show that the band type of the PCR amplification product of the pepper to be tested is all band type A (showing a band, which is 176bp), ...

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Abstract

The invention discloses a functional molecular marker of related genes of cell nuclear male sterility of peppers and application thereof. A specific primer pair provided by the invention consists of two primers for amplifying specific DNA fragments; the specific DNA fragments have target sequences of the primer pairs formed by primers InDel-12F and primers InDel-12R in a pepper genome; the primersInDel-12F is single-chain DNA molecules shown in a sequence 3 of a sequence table, and the primers InDel-12R is single-chain DNA molecules shown in a sequence 4 of the sequence table. By applicationof the molecular marker InDel-12 provided by the invention, the varieties of pepper with cell nuclear male sterility can be rapidly screened. The functional molecular marker and the application have important theoretical significance and economic value.

Description

technical field [0001] The invention belongs to the field of plant molecular breeding, and in particular relates to a functional molecular marker of a gene related to male sterility in pepper cells and an application thereof. Background technique [0002] Pepper (Capsicum annuum L.) is a herbaceous plant belonging to the family Solanaceae (Solanaceae), also known as pepper, sea pepper, spicy eggplant, pepper, etc. According to statistics, the global planting area of ​​pepper has exceeded 50 million mu, with an annual output of 37 million tons. It is the largest seasoning crop in the world. In my country, more than 20 million mu of pepper is planted, accounting for 35% of the world's pepper area and 10% of the national vegetable area, second only to the national Chinese cabbage planting area. The total economic output value of pepper exceeds 70 billion yuan, and its output value and benefit rank first among vegetable crops (China Vegetable Circulation Association). At prese...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172
Inventor 沈火林程青
Owner CHINA AGRI UNIV
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