Detection method of radix angelicae dahuricae coumarins in veterinary drugs

A detection method, the technology of coumarin, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of short life of liquid chromatography columns, large analysis errors, and low extraction accuracy of coumarin from Angelica dahurica, and achieve improved stirring and crushing effect, reduction in usage, effect of lowering temperature

Active Publication Date: 2018-06-12
宋瑞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The existing experimental process is not very accurate for the extraction of Angelica dahurica coumarin in veterinary medicine, which will lead to large analysis errors, and it is necessary to use a guard column to protect the liquid chromatography column, otherwise the life of the liquid chromatography column will be short

Method used

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  • Detection method of radix angelicae dahuricae coumarins in veterinary drugs
  • Detection method of radix angelicae dahuricae coumarins in veterinary drugs
  • Detection method of radix angelicae dahuricae coumarins in veterinary drugs

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Embodiment 1

[0030] A detection method of Angelica dahurica coumarin in veterinary medicine, comprising the following steps:

[0031] A. Add the veterinary drug to be tested in the pulverizing mechanism, then inject 60wt% ethanol aqueous solution, and stir and pulverize;

[0032] B, filter the aqueous ethanol solution containing the veterinary drug components to obtain the filtrate;

[0033] C, filtrate is added ether to extract;

[0034] D. Use a liquid chromatography column to separate the components of the extract, and then measure the coumarin content of Angelica dahurica through chromatographic analysis.

[0035] In step D, using a liquid chromatography column to separate the components of the extraction extract includes the following steps,

[0036] D1. Use Shimadzu InertSustain AQ-C18 liquid chromatography column, mobile phase: water, acetonitrile, dichloromethane, 2,2,2-trifluoroethyl methyl ether, flow rate: 2.5mL / min, injection volume: 10 μL;

[0037] D2, carry out gradient e...

Embodiment 2

[0044] This embodiment is improved on the basis of Embodiment 1. In Step A, the stirred solution included 55 wt% ethanol, 10 wt% glycerol and 35 wt% water. By adding glycerol, the pulverization and dissolution rate of the veterinary drug can be further improved to reduce the amount of filter residue in step B, thereby reducing the impact of coumarin contained in the filter residue on the accuracy of subsequent detection steps.

Embodiment 3

[0046]This embodiment is improved on the basis of Embodiment 2. The crushing mechanism includes a casing 1, the top of the casing 1 is provided with a first motor 2, the first motor 2 is connected with a first stirring paddle 3, the bottom of the casing 1 is provided with a second motor 4, and the second motor 4 is connected with a second stirring Paddle 5, the edge of casing 1 is provided with first columnar orifice 6 and second columnar orifice 7, and second columnar orifice 7 is positioned at the outside of first columnar orifice 6, and first columnar orifice 6 is connected with the first motor 2, and the second cylindrical orifice plate 7 is connected with the second motor 4. The first motor 2 and the second motor 4 rotate in opposite directions. Several first diversion grooves 8 are evenly arranged on the liquid-facing surface of the first stirring paddle 3, and the included angle between the first diversion groove 8 and the axial direction of the first stirring blade 3 ...

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Abstract

The invention discloses a detection method of radix angelicae dahuricae coumarins in veterinary drugs. The detection method comprises the following steps: A, adding a veterinary drug to be detected into a pulverizing mechanism, then injecting a 60wt% ethanol water solution and performing stirring and pulverizing; B, filtering the ethanol water solution containing the veterinary drug component to obtain filtrate; C, adding the filtrate to ether and performing extraction; and D, performing component separation on the extraction product by using a liquid chromatography column, and then determining the content of the radix angelicae dahuricae coumarins by chromatographic analysis. The detection method of radix angelicae dahuricae coumarins in the veterinary drugs can improve the defects of theprior art, improve the extraction accuracy of the radix angelicae dahuricae coumarins in the veterinary drugs, improve the accuracy of detection results and prolong the service life of the liquid chromatography column.

Description

technical field [0001] The invention relates to the technical field of veterinary drug detection, in particular to a method for detecting coumarin of Angelica dahurica in veterinary drugs. Background technique [0002] Angelica dahurica coumarin is one of the main active ingredients in Angelica dahurica, which has anti-inflammatory, labor pain and anti-histamine effects. The detection of Angelica dahurica coumarin content in veterinary medicine can be used as an important index for the detection of active ingredients in veterinary medicine. Currently, the detection of Angelica dahurica coumarin is generally carried out by chromatographic analysis (for example, Chinese invention patent CN 101637486 B). Chromatographic analysis is a high-precision analysis method. Before chromatographic analysis, the sample needs to be pulverized, Extract and separate the components of the analytical sample using a liquid chromatographic column. The existing experimental process does not hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/062
Inventor 宋瑞何立欢吕洁何立宁东贤赵兴鑫曹楠刘冰崔荣飞张梦雪
Owner 宋瑞
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