A traditional Chinese medicine composition for preventing and treating cardiorenal syndrome
A technique for cardio-renal syndrome and composition, applied in the field of preparation of the traditional Chinese medicine composition, can solve the problem of not recommending long-term use and the like, and achieve the effects of no toxic and side effects, improved quality of life, and remarkable therapeutic effect
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[0028] Use experimental data to show that the Chinese medicine composition of the present invention prevents and treats cardiorenal syndrome in vivo
[0029] 1. Test drug
[0030] Weigh the 7-day amount of the crude drug in proportion, add 18 times the amount of 50% ethanol to reflux and extract 3 times, each time for 1.5 hours. Filter, combine the extracts, and concentrate under reduced pressure to the equivalent of 1 g crude drug per ml.
[0031] 2. Experimental animals
[0032] Wiatar rats, male (200-250g), provided by Shanghai Lingchang Biotechnology Co., Ltd. (SCXK (Shanghai) 2013-0018).
[0033] 3. Experimental method
[0034] 3.1 Establishment of cardiorenal syndrome rat model
[0035] Rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (0.3 mL•100g-1) and fixed on the operating table. Turn on the ventilator (respiratory frequency 72 times min-1, tidal volume 18mL, respiratory ratio 1: 2), and insert a tracheal tube from the mouth of the rat....
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