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Method for determining content of total flavonoids in Ginkgo biloba leaves

A technology of ginkgo biloba leaves and total flavonoids, which is applied in the field of chemical detection, can solve problems such as systematic reports on the determination of total flavonoids content, and achieve the effects of increasing capacity, increasing diffusion, and reducing blocking

Inactive Publication Date: 2018-06-15
WUZHOU INST FOR FOOD & DRUG CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] But now there is no systematic report on the determination of the total flavonoids in Ginkgo biloba

Method used

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  • Method for determining content of total flavonoids in Ginkgo biloba leaves
  • Method for determining content of total flavonoids in Ginkgo biloba leaves
  • Method for determining content of total flavonoids in Ginkgo biloba leaves

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] 1 Instruments and reagents

[0035] 1.1 Instruments:

[0036] Electronic analytical balance (XA205DU), ASE350 fast solvent extractor (DIONEX, USA), ThermoU3000UHPLC liquid chromatograph

[0037] 1.2 Reagents:

[0038] Unless otherwise specified, all reagents used in this experiment were of analytical grade

[0039] Water: Class I water in accordance with GB / T 6682;

[0040] Ethanol (CH4O): chromatographically pure (for liquid chromatography).

[0041] 2 methods

[0042] 2.1 Preparation of reference solution:

[0043] Take an appropriate amount of quercetin reference substance, kaempferin reference substance, and isorhamnetin reference substance, accurately weigh them, and add ethanol to make a mixed solution containing 30 μg of quercetin, 30 μg of kaempferin, and 20 μg of isorhamnetin per 1 ml , that is.

[0044] 2.2 Preparation of the test solution

[0045] 2.2.1 "Chinese Pharmacopoeia" 2015 Edition Part I Preparation method for the test product:

[0046] Abou...

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Abstract

The invention discloses a method for determining content of total flavonoids in Ginkgo biloba leaves, belongs to the field of chemical detection, and aims to provide a good-reproducibility, few-interference-factor convenient and quick ASE-HPLC method for determining the content of the total flavonoids in Ginkgo biloba leaves. The method uses an ASE extraction method to extract the Ginkgo biloba leaves respectively with n-hexane and ethanol, and after an ethanol extraction solution is collected, the ethanol extraction solution is treated with hydrochloric acid to obtain an ethanol extract; anda HPLC method is used for determining the content of the total flavonoids in the ethanol extract. The method can replace the method in the pharmacopoeia for extracting and determining the total flavonoids in the Ginkgo biloba leaves.

Description

technical field [0001] The invention belongs to the field of chemical detection, in particular to a method for measuring the content of total flavonoids in ginkgo leaves. Background technique [0002] Ginkgo biloba is the dry leaf of Ginkgo biloba L. Harvest in autumn when leaves are still green and dry in time. Generally cultivated artificially. The cultivation area extends from Liaoning in the north, to Guangdong in the south, from Zhejiang in the east, to Shaanxi and Gansu in the west, and to Sichuan, Guizhou, and Yunnan in the southwest. [0003] It has the effects of promoting blood circulation and removing blood stasis, dredging collaterals and relieving pain, astringing the lungs and relieving asthma, and reducing turbidity and lipids; it is used for blood stasis blocking collaterals, chest obstruction and heartache, stroke hemiplegia, lung deficiency cough and asthma, hyperlipidemia. [0004] The main pharmacological active ingredients of Ginkgo biloba are flavono...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 张云平王华
Owner WUZHOU INST FOR FOOD & DRUG CONTROL