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A microsatellite marker linked to high fecundity traits of Procambarus clarkii and its application

A technology of microsatellite markers and Procambarus clarkii, applied in the field of animal molecular genetics and breeding, to speed up the progress of breeding, overcome low efficiency and reduce investment

Active Publication Date: 2021-08-17
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the link between microsatellite markers and high oviposition of Procambarus clarkii at home and abroad

Method used

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  • A microsatellite marker linked to high fecundity traits of Procambarus clarkii and its application
  • A microsatellite marker linked to high fecundity traits of Procambarus clarkii and its application
  • A microsatellite marker linked to high fecundity traits of Procambarus clarkii and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] 1. Take 101 Procambarus clarkii with eggs from the Procambarus clarkii farm in Yancheng City, Jiangsu Province, weigh the weight of each Procambarus clarkii, count the amount of eggs held by each Procambarus clarkii, and calculate the eggs of each Procambarus clarkii The ratio of number to weight. Sorting according to the ratio results, take the 24 individuals with the smallest relative egg holding capacity to form a low-fecundity group. A high-fecundity group is formed, and the value of the relative egg mass of each individual of the group is greater than 10 (egg grain / gram body weight). The tissue of Procambarus clarkii was collected, and the DNA of Procambarus clarkii was extracted, and the extraction method adopted the traditional phenol-chloroform extraction method. Specific steps are as follows:

[0030] 1.1 Put 0.1g muscle from each individual into a 1.5ml centrifuge tube, cut it into pieces, add 450μL STE extraction buffer (10mmol / L Tris-HCl, pH8.0; 1mmol / L ED...

Embodiment 2

[0043] 1. In October 2016, 2,000 Procambarus clarkii parents with eggs were collected from a Procambarus clarkii farm in Yancheng City, Jiangsu Province. The individuals were healthy, growing well, with complete appendages. Use a tightenable plastic collar with a numbered number to buckle at the base of the claws of the Procambarus clarkii to number each brooding shrimp;

[0044] 2. For the fertilized eggs of Procambarus clarkii attached to the appendages of the mother's abdomen at different developmental stages, use sterile tweezers to pick up 5-10 fertilized eggs (a total of 15-30 eggs) in the front, middle and back of the abdomen. In eppendorf tubes, quick-frozen in liquid nitrogen and brought back to the laboratory for temporary storage in a -80°C refrigerator;

[0045] 3. The genomic DNA of the embryos of Procambarus clarkii 2000 at different developmental stages was extracted using Soil Genomic DNA Kit from Beijing Kangwei Century Bioreagent Co., Ltd.

[0046] 4. Adopt ...

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Abstract

The invention discloses a microsatellite marker linked with the high fecundity trait of Procambarus clarkii and its application, belonging to the field of molecular genetic breeding of aquatic animals. The invention successfully obtains 2 pairs of microsatellite primer pairs, and obtains 2 microsatellite loci highly linked with high fecundity traits of Procambarus clarkii. Tightly linked molecular markers can speed up the breeding process and improve the accuracy of selection. Selecting individuals with linked markers for high fecundity traits of Procambarus clarkii as parents will greatly speed up the progress of breeding and obtain genotypes with high fecundity traits. A new species of Procambarus chinensis.

Description

technical field [0001] The invention relates to a microsatellite marker linked with the high fecundity trait of Procambarus clarkii and its application, belonging to the field of animal molecular genetic breeding. Background technique [0002] Procambarus clarkii (Procambarus clarkii), commonly known as crayfish, belongs to the phylum Arthropoda in animal taxonomy, Crustacea, Decapod order, Crayfish suborder, Cricketidae, and the genus Procambarus. The shrimp is native to North America and was introduced into Nanjing, Jiangsu and Chuzhou, Anhui through Japan in 1929. After decades of natural migration, reproduction and human breeding activities, it has been widely distributed in rivers, lakes, reservoirs, ponds, ditches, rice fields and other natural waters in most provinces and cities in my country, and has become an important freshwater economic shrimp in my country. one. [0003] With the expansion of the breeding area, the shortage of Crayfish seedlings is becoming more...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888C12Q1/686C12N15/11
CPCC12Q1/686C12Q1/6888C12Q2600/124C12Q2600/156C12Q2525/151
Inventor 沈怀舜胡亚成戴天豪黄鹏丹
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI