Method for detecting microplastics in organism

A technology of microplastics and organisms, applied in the field of environmental pollutant detection, can solve the problems of marine plastic pollution, aggravate environmental pollution, and difficult to degrade, and achieve the effect of accurate qualitative and quantitative detection

Active Publication Date: 2018-06-19
GUANGDONG OCEAN UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Due to its low cost, stable chemical properties, and good impact resistance, plastics are widely used in daily...

Method used

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  • Method for detecting microplastics in organism
  • Method for detecting microplastics in organism
  • Method for detecting microplastics in organism

Examples

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Embodiment 1

[0034] Example 1: Detection of microplastics in oysters

[0035] 1.1 Pretreatment of oysters

[0036] (1) A plurality of oysters caught in Zhanjiang waters were taken out from the refrigerator to thaw, the shells were removed, and washed with water.

[0037] (2) Weigh the cleaned oysters on an electronic balance, and record the weights respectively.

[0038] 1.2 Exploration of experimental conditions for digestion

[0039] The following methods are adopted for the digestion of oysters: enzymatic digestion, nitric acid and 30% hydrogen peroxide digestion, nitric acid digestion, saturated sodium chloride layering method and ethanol layering method.

[0040] 1.2.1 Enzymatic digestion method

[0041] (1) Take 5 oysters (114g) out of the refrigerator, and after pretreatment according to step 3.1, use a juice extractor (JYL-C52V multifunctional juice extractor, Joyoung Co., Ltd.; the same below) to crush them into pulp, add After 500 mL of water and 5 g of papain (papain, Guangz...

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Abstract

The invention relates to a method for detecting microplastics in an organism. The method comprises steps as follows: S110, digestion: an organism sample is soaked in nitric acid with the concentrationof 50%-70% at the room temperature for 6-18 h, heated to 60-95 DEG C and maintained for 1-4 h, and a digested organism sample is obtained; S120, degreasing: the organism sample obtained in S110 is washed with a surfactant at the temperature of 60-95 DEGC and subjected to suction filtration while hot, and degreased microplastics are obtained; S130, the degreased microplastics obtained in S120 arewashed with water at the temperature of 60-95 DEGC and subjected to suction filtration, and the cleaned microplastics are obtained. By means of the method, qualitative and quantitative detection can be rapidly and accurately performed on the microplastics entering the organism, the purpose of enrichment and distribution of the microplastics in the organism is achieved, and a practical tool is provided for environment monitoring.

Description

technical field [0001] The invention belongs to the field of environmental pollutant detection, and in particular relates to a method for detecting microplastics in organisms, in particular to a method for detecting microplastics in organisms by using a fluorescent microscope counting method. Background technique [0002] Due to its low cost, stable chemical properties, and good impact resistance, plastics are widely used in daily life, but because they are difficult to degrade, they aggravate environmental pollution, especially marine plastic pollution. Plastic pollutants in seawater are gradually degraded into tiny particles by factors such as light, heat, and biology, which are called microplastics. Once the microplastic pollution in water increases, it will cause toxicological reactions to marine organisms, such as inducing genetic aberrations, reproductive abnormalities, hormonal disorders, or toxicological changes in tissues, etc. Studying the composition and content ...

Claims

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Application Information

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IPC IPC(8): G01N1/30G01N1/34G01N1/40G01N15/14G01N21/3563G01N23/2202
CPCG01N1/30G01N1/34G01N1/4044G01N15/14G01N21/3563G01N23/2202G01N2001/302G01N2021/3595G01N2021/3572G01N2015/1486
Inventor 李承勇闫协民吕露露孙省利千忠吉周春霞洪鹏志
Owner GUANGDONG OCEAN UNIVERSITY
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