Method for selectively breeding excellent strains of saline-alkaline tolerant tilapia

A salt-alkali-resistant and tilapia-resistant technology is applied in fish farming, agricultural fishing, and climate change adaptation. Effects of genetic diversity, broad salt tolerance, avoidance of inbreeding

Active Publication Date: 2018-06-29
SHANGHAI OCEAN UNIV
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Problems solved by technology

[0004] In the process of developing excellent strains of saline-alkali-tolerant tilapia, there are problems such as poor operational feasibility, low breeding efficiency, and relatively simple adaptation to saline-alkaline environments, which affects production and promotion.
Among them, the salt-alkali tolerance breeding technology has the following disadvantages: 1) It is mainly a technical route formed for salinity stress, and the new tilapia varieties bred are also mainly salt-tolerant. It is possible to demonstrate farming in a wider area to maximize the fish farming industry
However, in reality, the environmental factors of water bodies in different aquaculture areas are very different, especially in saline-alkali water bodies, where the two stress factors of salinity and alkalinity exist at the same time, the impact on fish growth is more complicated, and the single salt-tolerant breeding technology and The variety is not suitable for wides

Method used

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  • Method for selectively breeding excellent strains of saline-alkaline tolerant tilapia
  • Method for selectively breeding excellent strains of saline-alkaline tolerant tilapia
  • Method for selectively breeding excellent strains of saline-alkaline tolerant tilapia

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1 Salt-alkali tolerance breeding experiment

[0039] as attached figure 1 As shown, the above-mentioned Nile tilapia is selected for a generation of salt-alkali tolerance every year. The specific process is preparation of breeding ponds, selection and cultivation of broodstock, hatching and seedling removal, screening, intensive cultivation and overwintering, specifically:

[0040] The first step, breeding pond preparation: choose a clear pond as the breeding pond, clean and disinfect, then inject new water and prevent wild fish and harmful organisms from entering the pond with the water, and apply fertilizer 7 days before broodstock enter the pond to make the water fertilized , to cultivate bait organisms in the water.

[0041] The second step, broodstock selection and cultivation: select mature broodstock and feed and cultivate them in the above-mentioned prepared breeding pond in mid-March. The water temperature in the pond is controlled above 23°C, and t...

Embodiment 2

[0045] Example 2 Comparison experiment of different strains of Nile tilapia daily weight gain in different saline-alkaline environments

[0046]Three different strains of Nile tilapia juveniles from Shanghai, Shandong and Hebei were selected and cultured in cages under different salinity, alkalinity and saline-alkaline mixed gradients.

[0047] The configuration method of different saline-alkali mixing gradients in the saline-alkali mixing group is as follows: first blend brine and fresh water into different salinities, and then add analytically pure NaHCO in a certain proportion 3 Each saline-alkali gradient was prepared, and the upper layer of clear water was taken out after 2 days of precipitation for testing. Among them, SG7 portable salinity meter and HI83200 multi-parameter water quality analyzer were used to detect salinity and alkalinity respectively.

[0048] The Shanghai strain is in the single salinity 15‰ group and the single salinity 20‰ group; the Shandong strain...

Embodiment 3

[0049] Embodiment 3 evaluates and monitors the saline-alkaline tolerance experiment of different strains of Nile tilapia

[0050] While carrying out salt-alkali tolerance breeding for the above-mentioned Nile tilapia, evaluate and monitor its salt-alkali tolerance, including growth experiments, acute lethal experiments and chronic lethal experiments, specifically:

[0051] The growth experiment was carried out in a cement pool, and five groups were first set up, namely fresh water group, single salinity group (10‰), saline-alkali mixed group (salinity 10‰, alkalinity 2g / L), saline-alkali mixed group (salinity degree 10‰, alkalinity 4g / L) and saline-alkali mixed group (salinity 10‰, alkalinity 6g / L). Before the experiment, mark the left and right pectoral fins of the fish used in the experiment to distinguish the breeding group and the control group. There are 240 fish in each pond, and 120 fish in the breeding group and the control group. The weight, body length and body heigh...

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Abstract

The invention discloses a method for selectively breeding excellent strains of saline-alkaline tolerant tilapia. The method includes steps of collecting at least three different strains of Nile tilapia to be used as selective breeding base population and evaluating the saline-alkaline tolerance; carrying out one-generation saline-alkaline tolerant selective breeding on the different strains of Nile tilapia in every year and continuously selectively breeding at least two generations; mixing the different strains of saline-alkaline tolerant Nile tilapia with obvious growth superiority with one another after saline-alkaline tolerant selective breeding is carried out on the different strains of saline-alkaline tolerant Nile tilapia, then carrying out saline-alkaline tolerant selective breedingon the different strains of saline-alkaline tolerant Nile tilapia and continuously selectively breeding at least four generations to obtain the saline-alkaline tolerant Nile tilapia of the excellentstrains. Saline-alkaline tolerant selective breeding procedures include reproduction pond preparing, parent tilapia selecting and rearing, fry hatching and fishing, screening and intensified culture and overwintering. The method has the advantages that the method is high in reliability, operability and efficiency; novel technical ways can be developed for selectively breeding stress-resistant varieties of fish, and the method has important significance and application value in popularization of rearing of stress-resistant improved varieties of cultured fish.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a method for breeding an excellent strain of saline-alkali-tolerant tilapia. Background technique [0002] At present, the available fresh water resources for fishery production in my country are shrinking day by day, and there are about 31 million hm 2 In saline-alkali waters, the biological resources in most of the water bodies are relatively poor and basically idle. Rational use of saline-alkali water for aquaculture can transform the unconventional resource of saline-alkali water into regional characteristic economic advantages, while protecting and improving the environment , wherein the development of varieties suitable for saline-alkaline aquaculture is an important way to achieve this goal. [0003] Nile tilapia (Oreochromis niloticus) has excellent characteristics such as fast growth, delicious taste and few spines. It is an important breeding species fo...

Claims

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Application Information

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IPC IPC(8): A01K61/10
CPCA01K61/10Y02P60/60Y02A40/81
Inventor 赵金良赵岩筴金华张艳红任炳琛
Owner SHANGHAI OCEAN UNIV
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