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Application of volatile substance produced by streptomyces fimicarius in control of plant diseases

A technology for plant disease control and volatile substances, applied in the fields of application, biocide, plant growth regulator, etc., can solve the problem of single antibacterial function

Active Publication Date: 2018-06-29
HAINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The invention provides an application of volatile substances produced by Streptomyces faecalis in the prevention and control of plant diseases, which solves the problem of single function of single biocontrol bacteria in the prior art

Method used

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  • Application of volatile substance produced by streptomyces fimicarius in control of plant diseases
  • Application of volatile substance produced by streptomyces fimicarius in control of plant diseases
  • Application of volatile substance produced by streptomyces fimicarius in control of plant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1, the preparation of volatile substances produced by Streptomyces faecalis BWL-H1

[0032] Streptomyces faecalis BWL-H1 strain was streak cultured on a PDA plate for 7 days, scraped fresh spores into a 250 mL Erlenmeyer flask containing 100 mL of ISP2 medium, and then placed on a shaker at 200 rpm for 3 days at 28°C to prepare spore suspension Liquid (about 1×10 7 spores / mL), inoculated on sterile wheat grain medium at the ratio of 1mL / 100g (V / W). After shaking well, culture at 25°C for 21 days, during which the wheat grain medium was shaken once every 3 days, so that the strain BWL-H1 could grow evenly.

Embodiment 2

[0033] Embodiment 2, the bacteriostatic spectrum determination of the volatile matter produced by Streptomyces faecalis BWL-H1

[0034] Inoculate the pathogenic bacteria to be tested on the PDA medium, culture in a constant temperature incubator at 25°C for 6 days, and then use a puncher to Get bacterium cake at the bacterium colony outer edge, place fresh PDA plate center, take by weighing 20g / L Streptomyces faecalis BWL-H1 wheat kernel culture (preparation method is referring to embodiment 1) and move in 90mm culture dish, then two dishes The bottoms are buckled together, and finally the two petri dishes are tightly sealed together with a sealing film to make a double-dish interlocking device. The pathogenic bacteria are on the top and the wheat culture is on the bottom, which can prevent Streptomyces faecalis BWL- When H1 is in contact with pathogenic bacteria, the gas between them can fully flow, and 20g / L of sterile wheat grains is used as a control. Place the double-di...

Embodiment 3

[0039] Embodiment 3, the impact of culture time on the antibacterial activity of volatile substances produced by Streptomyces faecalis BWL-H1

[0040] Place four small petri dish bottoms in a large Petri dish with a diameter of 150mm and a height of 30mm (total internal volume 500mL) Among them, 3 small petri dishes contained about 5mL of PDA medium, and a piece of Pythophthora litchia cake was inoculated in the center of the medium Another small petri dish is placed 16g / L Streptomyces faecalis BWL-H1 grain culture (respectively cultivated 4d, 7d, 14d, 21d, 28d), with the aseptic wheat grain of 16g / L as contrast, then large Petri dishes are sealed. Incubate at a constant temperature of 25°C for 6 days, measure the colony diameter and calculate the bacteriostatic rate. Each treatment was replicated 3 times, and the experiment was repeated 3 times. see results figure 1 .

[0041] The results showed that different culture time had significant effect on the production of vola...

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Abstract

The invention discloses an application of a volatile substance produced by streptomyces fimicarius in control of plant diseases. Particularly, the volatile substance produced by streptomyces fimicarius can inhibit growth of 15 phytopathogens, has the highest inhibition function on peronophythora litchii, pythium myriotylum, alternaria alternate, fusarium graminearum, colletotrichum musarum and pyricularia grisea and has the inhibition rate up to 100%. The invention further makes clear that the volatile substance produced by streptomyces fimicarius contains 7 compounds with remarkable bacteriostasis, and a direction is provided for future studies of control drugs for peronophythora litchii, pythium myriotylum, alternaria alternate, colletotrichum musarum, pyricularia grisea and other phytopathogens.

Description

technical field [0001] The invention relates to the field of biological control of plant diseases, in particular to the application of volatile substances produced by Streptomyces faecalis in the control of plant diseases. Background technique [0002] Plant diseases are one of the important factors affecting the benefits of agricultural products. At present, chemical drugs are mainly used to prevent and control plant diseases. However, chemical drugs have a series of problems such as strong toxic and side effects, easy residues, environmental pollution, and drug resistance. The use of chemical drugs is increasing. more restricted. The use of biocontrol bacteria or their metabolites to control plant pathogenic bacteria has the advantages of short cycle, low toxicity, no pollution and convenient production, and is an effective way for green prevention and control of agricultural products. [0003] However, the current use of a single biocontrol bacterium for biological contr...

Claims

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Application Information

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IPC IPC(8): A01N63/02A01N31/04A01N31/08A01N27/00A01N37/10A01N37/40A01N37/44A01P3/00
CPCA01N27/00A01N31/04A01N31/08A01N37/10A01N37/40A01N37/44A01N63/10
Inventor 邢梦玉张荣意刘铜
Owner HAINAN UNIVERSITY
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