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Substrate for detecting N-acetamido-2-deoxy-beta-D-glucopyranosidase, preparation method thereof and kit

A technology of glucopyranoside and glucopyranose, applied in the preparation of sugar derivatives, sugar derivatives, sugar derivatives, etc., can solve the problems of urine color confusion, weak positive missed detection, color insensitivity, etc. It is not easy to miss detection, the synthesis process is simple, and the color rendering is sensitive to the effect of missed detection.

Inactive Publication Date: 2018-06-29
GUANGDONG UNITY BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, most of them now use 4-nitrophenyl-2-acetylamino-2-deoxy-β-D-glucopyranoside as a substrate to detect N-acetylamino-2-deoxy-β-D-glucopyranose Glucosidase, this substrate mainly has the following problems: 1. The color development is not sensitive, and weak positives are easily missed; 2. The color development is yellow, which is easy to confuse with the color of urine and is difficult to distinguish

Method used

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  • Substrate for detecting N-acetamido-2-deoxy-beta-D-glucopyranosidase, preparation method thereof and kit
  • Substrate for detecting N-acetamido-2-deoxy-beta-D-glucopyranosidase, preparation method thereof and kit
  • Substrate for detecting N-acetamido-2-deoxy-beta-D-glucopyranosidase, preparation method thereof and kit

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Embodiment Construction

[0019] The present invention is illustrated below through specific examples, but the present invention is not limited.

[0020] Thymolphthalein, 2-acetylamino-3,4,6-tri-O-acetyl-2-deoxy-A-D-glucopyranosyl chloride and other raw materials can be commercially available.

[0021] 1. Synthesis of thymolphthalein-N-acetyl-3,4,6-O-triacetyl-β-D-glucopyranoside. It can be represented by the following reaction formula:

[0022]

[0023] The specific preparation steps are as follows: Thymolphthalein (4.3g, 10mmol), TBAHS (3.4g, 10mmol), 2-acetylamino-3,4,6-tri-O-acetyl-2-deoxy-A-D-pyridine Glucopyranoyl chloride (3.65g, 10mmol) was dissolved in 30ml of dichloromethane and cooled to 10°C, 30ml of 1N potassium carbonate aqueous solution was added and stirred at room temperature for 3 hours, separated and dried over anhydrous sodium sulfate. The organic phase was concentrated to dryness and passed through the column to obtain thymolphthalein-N-acetyl-3,4,6-O-triacetyl-β-D-glucopyrano...

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Abstract

The invention discloses a substrate for detecting N-acetamido-2-deoxy-beta-D-glucopyranosidase, and particularly discloses thymolphthalein-2-acetamido-2-deoxy-beta-D-glucopyranoside. A preparation method of the thymolphthalein-2-acetamido-2-deoxy-beta-D-glucopyranosidase comprises the following steps: reacting thymolphthalein with 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-A-D-glucopyranosyl chlorideto prepare thymolphthalein-N-acetyl-3,4,6-O-triacetyl-beta-D-glucopyranoside, and then removing acetyl groups to obtain the thymolphthalein-2-acetamido-2-deoxy-beta-D-glucopyranoside. The thymolphthalein-2-acetamido-2-deoxy-beta-D-glucopyranoside is used as the substrate for detecting the N-acetamido-2-deoxy-beta-D-glucopyranosidase, and has the advantages of sensitive development and low possibility of missed detection; in addition, the synthetic process is simple and the cost is reduced.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a substrate for detecting N-acetylamino-2-deoxy-β-D-glucopyranosidase and a preparation method thereof, and a substrate for detecting N-acetylamino-2 - A kit for deoxy-β-D-glucopyranosidase. Background technique [0002] N-acetylamino-2-deoxy-β-D-glucopyranosidase is an acidic hydrolase located in lysosomes with a molecular weight of about 140,000. It exists in all tissues, with the highest content in the prostate and kidney tubules. When the own tissue is damaged, especially when the proximal convoluted tubule is damaged, the activity of N-acetylamino-2-deoxy-β-D-glucopyranosidase in urine increases significantly, and it is higher than the increase of other urinary enzymes It appears early, so the in vitro detection of N-acetylamino-2-deoxy-β-D-glucopyranosidase in urine can provide a basis for early diagnosis and condition monitoring of renal tubular damage. [0003] Howev...

Claims

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Application Information

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IPC IPC(8): C07H1/00C07H15/26G01N21/78
Inventor 苏宏文赖华梁伟业刘志文
Owner GUANGDONG UNITY BIOTECH