Polypeptide serving as immune stimulus of B-cell epitope or peptide hapten for forming polypeptide immunogen and application of polypeptide

A peptide immunogen and B cell technology, applied in the direction of fusion peptides, antibody medical components, antibody mimics/scaffolds, etc., to improve immunogenicity, reduce content and/or activity, and improve symptoms

Inactive Publication Date: 2018-06-29
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The determination of B cell epitopes or peptide haptens is of great significance to the synthesis of peptide vaccines, the preparation of diagnostic reagents, and the screening of monoclonal antibodies. The premise is to have stro

Method used

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  • Polypeptide serving as immune stimulus of B-cell epitope or peptide hapten for forming polypeptide immunogen and application of polypeptide
  • Polypeptide serving as immune stimulus of B-cell epitope or peptide hapten for forming polypeptide immunogen and application of polypeptide
  • Polypeptide serving as immune stimulus of B-cell epitope or peptide hapten for forming polypeptide immunogen and application of polypeptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1: A polypeptide IA2(5)-P2, IA-2(5)-P2-1, IA-2(5)-P2-2 and IA- Preparation and synthesis of 2(5)-B-P2-1 and others.

[0047] A linear B cell epitope IA2(5) peptide of IA-2 is selected, the amino acid sequence 1 is shown in SEQ ID NO.1, the Th2 epitope P2 peptide at the C-terminal of P277 is selected, and the amino acid sequence 2 is shown in SEQ ID NO.2. The analysis software and a large number of STZ diabetes model mouse experiments were screened, analyzed, identified and modified, and the two epitopes were determined to be connected directly or with a flexible peptide, and the C-terminal of the IA2(5) peptide FEYQD and the N of the Th2 epitope P2 peptide were determined. IA2(5)-P2, IA-2(5)-P2-1IA-2(5)-P2-2, etc. are formed directly or through the connection of flexible peptides or connecting peptides. The amino acid sequences of flexible peptide connecting peptides are shown in SEQ ID NO.3 and SEQ ID NO.4. The amino acid sequences of IA2(5)-P2, IA-2(5)-P2-1I...

Embodiment 2

[0048] Example 2: Preparation and synthesis of B cell epitope or synthetic peptide hapten B.

[0049] Using B cell antigen epitope analysis software and online analysis tools, based on a single parameter, combined with secondary structure prediction and spatial structure characteristics, respectively from a target antigen protein (such as: DPP4 or XOD or HMG-CoA reductase and uric acid transporter Multiple dominant B cell antigen epitopes or peptide hapten B were selected from more than 100 epitopes of protein 1), and combined with IA2(5)-P2, IA2(5)-P2-1, IA2(5)-B -P2-1, etc. are connected with flexible peptides, and through immunization animal experiments to screen any re-engineering and re-screening processes to obtain highly immunogenic B cell antigen epitopes or synthetic peptide haptens B. All peptides were synthesized by FMOC solid-phase synthesis method, and the purity was >90% as detected by HPLC.

[0050] Example 2-1 When B is the dominant B cell epitope of human dip...

Embodiment 2-3

[0052] Example 2-3 When B is human HMG-CoA reductase (it is the rate-limiting enzyme in the process of liver cell synthesis of cholesterol, select H1, H2, H3 ----- etc. from more than 100 epitopes) Predominant B cell antigen epitope or peptide hapten B, which forms a peptide immunogen by connecting with the N-terminus of IA2(5)-P2, IA2(5)-P2-1, IA2(5)-P2-2 with a flexible peptide, The highly immunogenic B cell antigen epitope or peptide hapten H, H1, etc. were screened through immunization animal experiments. H and H1 are a specific polypeptide of xanthine oxidoreductase, and the amino acid sequence is shown in SEQ ID NO.13 and SEQ ID NO.13. ID NO.14.

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Abstract

The invention provides a polypeptide containing a T helper cell epitop (Th epitope), which can serve as an immune stimulus of a B-cell epitope or a peptide hapten for forming a polypeptide immunogen,and application of polypeptide. The B-cell epitope or the peptide hapten is covalently connected with polypeptide through a connecting peptide to form the polypeptide immunogen; the polypeptide-containing polypeptide immunogen is obtained through repeated screening and analyzing of mice, so that the immunogenicity of the B-cell epitope or the peptide hapten can be enhanced remarkably, an antibodywhich can be specifically bonded to a target antigen protein can be produced, the immunoregulation of biased Th2 can be enhanced remarkably, and symptoms can be improved. The polypeptide as well as polypeptide-containing immunogen, derivatives and analogues provided by the invention can also be used for recombinant microorganisms and animals or plants and cells which can be genetically modified, so that the recombinant microorganisms and the animals or the plants and the cells which can be genetically modified serve as a production factory to produce vaccines or prepare oral vaccines or produce specific antibodies and derivatives and modifiers for preventing and treating dominant diseases, including but not limited to diabetes and complications, rheumatoid arthritis and the like, and for developing testing agents.

Description

technical field [0001] The present invention relates to a polypeptide containing a T helper cell epitope (Th epitope) and its application, especially for use of the polypeptide with a B cell epitope or a peptide hapten---a target antigenic site of a target antigenic protein Covalently linked to become a new type of polypeptide immunogen, which can be used as an immunostimulator of B cell epitope or peptide hapten, enhance the immunogenicity of the target antigen site (B cell epitope or peptide hapten), and can be used to prepare specific and Target antigen protein-binding antibodies containing B cell epitopes or peptide immunogens, novel polypeptide immunogens containing the polypeptides, coupling proteins and fusion proteins, and specific antibodies produced based on them, as well as derivatives and analogs containing the polypeptides and Drugs and preparations of specific antibodies and antibody derivatives and analogs produced on the basis thereof have important application...

Claims

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Application Information

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IPC IPC(8): C07K19/00A61K39/00A61P3/10A61P19/06A61P13/12A61P3/06A61P9/10A61P19/02A61P29/00A61P25/28A61P27/02A61P9/00G01N33/68
CPCA61K39/00A61P3/06A61P3/10A61P9/00A61P9/10A61P13/12A61P19/02A61P19/06A61P25/28A61P27/02A61P29/00C07K19/00G01N33/68C07K14/4713A61K39/0008C07K14/47C07K2319/00
Inventor 李泰明顾小骞李志鑫方金芝魏晓敏孟凯特焦林芳马艳洁
Owner CHINA PHARM UNIV
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