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Method for improving silkworm parthenogenesis early generation hatching rate

A technology of parthenogenesis and hatching rate, which is applied in the field of insect genetic breeding, can solve the problems of reducing the hatching rate of silkworm eggs, protein denaturation and inactivation, etc., and achieve the effects of increasing hatching rate, reducing negative effects, and improving breeding efficiency

Active Publication Date: 2018-07-03
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

Although heat shock treatment at 46°C for 18 minutes can activate unfertilized eggs in division arrest to resume division, it also causes protein denaturation and inactivation, activates the apoptosis mechanism of silkworm eggs, and thus greatly reduces the hatching rate of silkworm eggs

Method used

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  • Method for improving silkworm parthenogenesis early generation hatching rate
  • Method for improving silkworm parthenogenesis early generation hatching rate

Examples

Experimental program
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Effect test

Embodiment 1

[0025] A method for improving parthenogenesis of silkworm, the method is carried out according to the following steps:

[0026] 1) Treatment of mature female pupae: After cocooning in the upper cocoons, after 10 days of routine protection, the cocoons were peeled to take pupae and identify male and female; healthy female pupae were poured into freshly prepared 0.25% bleach solution, soaked for 2 minutes, and removed from room temperature After drying, routinely protect until 1-2 days before eclosion;

[0027] 2) Inhibitor preparation and pupal injection: Dissolve the proteasome inhibitor MG132 powder in DMSO, prepare the inhibitor stock solution, and dilute it to an appropriate concentration with 1×PBS before use; inject 20 microliters per pupa into the abdomen of the female pupa , so that the final concentration of the inhibitor is 0.5-2 μmoL / L, and routinely protect to eclosion;

[0028] 3) Collection and cleaning of unfertilized eggs: pick the abdomen of virgin moths after...

Embodiment 2

[0032] In the step (1) of this example: the female pupae is routinely protected until 2 days before eclosion; in the step (2): the final concentration of the inhibitor MG132 after injection is 0.5 μmoL / L; in the step (4): heat shock induction for 18 minutes; Step process is the same as embodiment 1.

Embodiment 3

[0034] In the step (1) of this example: the female pupae is routinely protected until 2 days before eclosion; in the step (2): the final concentration of the inhibitor MG132 after injection is 1.33 μmoL / L; in the step (4): heat shock induction for 18 minutes; Step process is the same as embodiment 1.

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Abstract

The invention relates to the technical field of insect genetic breeding, and particularly relates to a method for improving silkworm parthenogenesis early generation hatching rate. The method is characterized in that old mature female pupas are injected with a proteasome inhibitor MG132 with a concentration of 0.5-2 [mu]mol / L 1-2 days before emergence; after emergence, abdomens of virgin moths aretaken to obtain unfertilized silkworm eggs; the unfertilized silkworm eggs are immersed in a water bath of 46 DEG C, and are heat shock induced for 15-18 min; and after the induction, the unfertilized eggs are protected at 16 DEG C for 3 days, then the bivoltine breed is hastened for hatching after immediate acid immersion, and the polyvoltine breed is directly hastened for hatching. According tothe method, the silkworm parthenogenesis induction method is improved, while induction of the unfertilized eggs to remove fission block and restore mitosis is maintained, the side effect that the hatching rate of silkworm eggs is decreased is relieved, thereby improving the parthenogenesis early generation hatching rate, and shortening a parthenogenesis breed breeding cycle.

Description

technical field [0001] The invention relates to the technical field of insect genetic breeding, in particular to a method for improving the hatching rate of the early generation of silkworm artificially induced parthenogenesis. Background technique [0002] Parthenogenesis in silkworm refers to the phenomenon that a female germ cell directly develops into a new individual. In the natural state, the frequency of its occurrence is very low, but artificial stimulation with physical and chemical factors can obtain a certain proportion of parthenogenetic eggs or larvae. Cytology proves that when parthenogenesis occurs in the silkworm, the germ cell chromosomes do not undergo meiosis, but only undergo mitosis, and the offspring completely replicate the mother's genotype. The research on parthenogenesis of the current silkworm varieties shows that the parthenogenesis traits of silkworm are quite different among different varieties. Genetic analysis shows that the parthenogenetic ...

Claims

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Application Information

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IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 翁宏飚牛宝龙杜鑫王永强
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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