Immunosuppressive macrolide antibiotic multistage loop biological fermentation reactor

Abstract

The invention relates to an immunosuppressive macrolide antibiotic multistage loop biological fermentation reactor. The reactor comprises a reactor shell (1), a multistage guide cylinder (2), a gas distributor (3), a temperature control jacket (4), a guide cylinder fixing plate (5) and feeding opening (6). The reactor allows sterile air to be injected into the reactor through the gas distributor,and a fluid in the guide cylinder forms a loop state; gas-liquid-solid three phases in the reactor are fully contacted, the mass transfer rate is high, the gas dissolution speed is fast, and a bottom-to-top gradient difference between a material and dissolved oxygen in the multistage guide cylinder provides different dissolved oxygen and nutrient requirements for microbes in different growth stages; solid floc microbes are evenly distributed in the reactor, and do not locally accumulate or deposit; and the heat transfer effect is good, the heat transfer efficiency between the material in the reactor and the wall of the reactor is high, and the temperature distribution of all parts of the reactor is uniform.

Description

technical field [0001] The invention relates to a multistage circulation biofermentation reactor, in particular to a circulation reactor which can be used for biofermentation production of immunosuppressive macrolide antibiotics, and belongs to the field of bioengineering. Background technique [0002] At present, macrolide antibiotics are mostly produced by biological fermentation, and the most widely used reactors are stirred fermenters and bubble column reactors. These two types of reactors are easy to operate, but generally have the disadvantage of low three-phase mass transfer efficiency, and the reactor is usually a homogeneous system, and the production of secondary metabolites usually requires different concentrations of dissolved oxygen and nutrients from the growth of bacteria Therefore, the efficiency of the above two types of reactors to produce macrolide antibiotics secondary metabolites with extremely low concentrations is relatively low. [0003] The internal...

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Problems solved by technology

[0004] The purpose of the present invention is: on the basis of traditional circulation bioreactor, develop a kind of multi-stage circulation biofermentation reactor for immunosuppressive macrolide antibiotics, adopt multi-stage guide tube, solve the problem of high transmission To solve the problem of creating different microenvironments in mass reactors, under the premise of ensuring high three-phase mass transfer efficiency, the same reactor provides a gradient environment for the growth of fermenting microorganisms at different growth stages, including dissolved oxygen and nutrients in the medium concentration, to prevent local accumulation or deposition of medium and biological cells and affect the fermentation of high-density strains, this invention can be widely used in the microbial fermentation production of secondary metabolites such as macrolide antibiotics

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