Kit and detection method of folic acid metabolic capability and genotyping as well as application of kit

Abstract

The invention relates to a folic acid metabolic capability and genotyping detection technology and in particular relates to a kit and a detection method of a folic acid metabolic capability and genotyping as well as application of the kit. The kit comprises specific primers aiming at 677C>T mutation and 1298A>C mutation of an MTHFR (Methylene Tetrahydrofolate Reductase) gene, and 66A>G mutation ofan MTRR (Methionine Synthase Reductase) gene, and a specific mutation detection probe, RT-PCR (Reverse Transcription-Polymerase Chain Reaction) buffer, an RT-PCR reaction Taq enzyme, sterile purifiedwater, a negative quality control product, a wild type quality control product, a mutant quality control product and a packaging box for separating and packaging a reagent bottle or pipe. The detection method of the folic acid metabolic capability and the genotyping, provided by the invention, has the advantages of strong specificity, high sensitivity, small pollution, simplicity and rapidness inoperation, high safety performance and the like; a detection result has relatively good accuracy and repeatability; the detection method is especially suitable for directly taking whole blood as a detection sample and related gene mutation detection is directly carried out; a genome DNA (Deoxyribonucleic Acid) template does not need to be extracted. The folic acid metabolic capability can be accurately judged and individualized folic acid oral administration and supplementing dosages are provided; the kit and the detection method have important value.

Description

technical field [0001] The invention relates to a folic acid metabolic ability genotyping detection technology, in particular to a kit, a detection method and an application thereof for the folic acid metabolic ability genotyping. Background technique [0002] Folic acid is a water-soluble vitamin found in spinach, also known as vitamin B9. Subsequently, scientists discovered that folic acid is contained in various animal and plant foods, and this type of folic acid is called natural folic acid. The human body can obtain folic acid supplements through the consumption of food, especially green leafy vegetables. In addition, you can also supplement folic acid by taking synthetic folic acid tablets, which has a higher bioavailability, which is about twice that of natural folic acid. Folic acid plays an important role in the division and growth of cells and the synthesis of nucleic acids, amino acids, and proteins, and is also an indispensable nutrient for fetal growth and dev...

AI Technical Summary

Problems solved by technology

[0007] However, in the existing technology, the fluorescent PCR method is used to detect the genotyping of folic acid metabolism ability, and it is necessary to extract blood genomic DNA as a template for corresponding detection. cost and time

In addition, the existing fluorescent PCR method to detect the genotype of folic acid metabolism capacity is mostly to set up a detection tube for each locus, such as detecting the MTHFR gene 677C>T mutation, MTHFR gene 1298A>C mutation and MTRR gene 66A>C mutation in a sample. There are three sites for G mutation, and three detection tubes need to be set up for corresponding detection. The operation is time-consuming and laborious, which is not conducive to large-scale clinical promotion and use.

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